The γTuRC components Grip75 and Grip128 have an essential microtubule-anchoring function in the Drosophila germline
The γTuRC components Grip75 and Grip128 have an essential microtubule-anchoring function in the Drosophila germline
复制标题
γTuRC 成分 Grip75 和 Grip128 在果蝇种系中具有重要的微管锚定功能
作者：
Nina Vogt;Iris Koch;H. Schwarz;F. Schnorrer;C. Nüsslein
γ-微管蛋白环复合物（γTuRC）是动物细胞中微管成核的重要模板。γ TuRC的分子组成已被描述;然而，拟形成帽结构的亚基的功能仍有待体内表征。在果蝇中，γTuRC的核心组分是有丝分裂所必需的，而帽组分Grip 75不是活力所必需的，但在卵子发生期间在bicoid RNA定位中起作用。尚未对其他帽组件进行体内分析。我们报告了帽组件Grip 128和Grip 75的功能表征。在Dgrip 128或Dgrip 75突变的动物是可行的，但男性和女性都是不育的。这两种蛋白质都是形成不同微管组所必需的，这有助于卵子发生过程中bicoid RNA的定位，形成连接卵母细胞减数分裂II纺锤体和雄性减数分裂中胞质分裂的中央微管aster。Grip 75和Grip 128锚轴丝在细胞核在精子伸长。我们建议，Grip 75和Grip 128需要系在特定的微管组织中心的微管，而不是需要一般的微管成核。γTuRC帽结构可能仅对基于非中心体的微管功能是必需的。
The γ-tubulin ring complex (γTuRC) forms an essential template for microtubule nucleation in animal cells. The molecular composition of theγ TuRC has been described; however, the functions of the subunits proposed to form the cap structure remain to be characterized in vivo. In Drosophila, the core components of the γTuRC are essential for mitosis, whereas the cap component Grip75 is not required for viability but functions in bicoid RNA localization during oogenesis. The other cap components have not been analyzed in vivo. We report the functional characterization of the cap components Grip128 and Grip75. Animals with mutations in Dgrip128 or Dgrip75 are viable, but both males and females are sterile. Both proteins are required for the formation of distinct sets of microtubules, which facilitate bicoid RNA localization during oogenesis, the formation of the central microtubule aster connecting the meiosis II spindles in oocytes and cytokinesis in male meiosis. Grip75 and Grip128 anchor the axoneme at the nucleus during sperm elongation. We propose that Grip75 and Grip128 are required to tether microtubules at specific microtubule-organizing centers, instead of being required for general microtubule nucleation. The γTuRC cap structure may be essential only for non-centrosome-based microtubule functions.
影响因子：
4
作者：
S. Endow;Rashmi Chandra;D. J. Komma;Akihiko H. Yamamoto;Edward D. Salmon
通讯作者：
S. Endow;Rashmi Chandra;D. J. Komma;Akihiko H. Yamamoto;Edward D. Salmon
影响因子：
3.3
作者：
Gunawardane, RN;Martin, OC;Zheng, YX
通讯作者：
Zheng, YX