Probabilistic transition from unstable predator-prey interaction to stable coexistence of Dictyostelium discoideum and Escherichia coli
Probabilistic transition from unstable predator-prey interaction to stable coexistence of Dictyostelium discoideum and Escherichia coli
复制标题
DOI：
10.1016/j.biosystems.2010.11.006
复制
发表时间：
2011-03-01
期刊：
影响因子：
1.6
通讯作者：
Yomo, Tetsuya
中科院分区：
文献类型：
--
作者：
Kihara, Kumiko;Mori, Kotaro;Yomo, Tetsuya
在生态系统的各个层面都发现了捕食者-猎物的相互作用。尽管它们在生态上的普遍存在和重要性，但向稳定共存状态过渡的过程却很少得到实验验证。为了研究捕食者-食饵相互作用的稳定过程，我们之前在盘状盘齿龙和大肠杆菌之间构建了一个可重复的实验捕食者-食饵系统，并表明表型改变的大肠杆菌有助于系统的稳定。在本研究中，我们重点研究了大肠杆菌表型改变后两种物种向稳定共存的过渡。从共培养板中分离的大肠杆菌细胞作为单个菌落进行分析，使我们能够很容易地识别出菌落形态和生长速度不同的表型改变大肠杆菌的外观。结果还表明，两种物种在空间分布上存在概率性差异，出现了稠密型和稀疏型两种粘性集落，且稳定维持的粘性集落均为稀疏型。这些结果表明，表型改变的大肠杆菌可能产生两种粘性菌落。空间分布的差异会影响两种物种之间的局部相互作用，从而导致捕食者-猎物相互作用的概率稳定。2010爱思唯尔爱尔兰有限公司版权所有。
Predator-prey interactions have been found at all levels within ecosystems. Despite their ecological ubiquity and importance, the process of transition to a stable coexistent state has been poorly verified experimentally. To investigate the stabilization process of predator-prey interactions, we previously constructed a reproducible experimental predator-prey system between Dictyostelium discoideum and Escherichia coli, and showed that the phenotypically changed E. coli contributed to stabilization of the system. In the present study, we focused on the transition to stable coexistence of both species after the phenotypic change in E. coli. Analysis of E. coli cells isolated from co-culture plates as single colony enabled us to readily identify the appearance of phenotypically changed E. coli that differed in colony morphology and growth rate. It was also demonstrated that two types of viscous colony, i.e., the dense-type and sparse-type, differing in spatial distribution of both species emerged probabilistically and all of the viscous colonies maintained stably were of the sparse-type. These results suggest that the phenotypically changed E. coli may produce two types of viscous colonies probabilistically. The difference in spatial distribution would affect localized interactions between both species and then cause probabilistic stabilization of predator-prey interactions. (C) 2010 Elsevier Ireland Ltd. All rights reserved.