The XRCC2 DNA repair gene from human and mouse encodes a novel member of the recA/RAD51 family
The XRCC2 DNA repair gene from human and mouse encodes a novel member of the recA/RAD51 family
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DOI：
10.1093/nar/26.13.3084
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发表时间：
1998-07-01
影响因子：
14.9
通讯作者：
Thacker, J
中科院分区：
文献类型：
--
作者：
Cartwright, R;Tambini, CE;Thacker, J
我们最近在人染色体7q36.1上发现了XRCC 2 DNA修复基因的一个位置候选者，我们现在已经从人和小鼠中克隆了该基因的cDNA，并表明它是recA/RAD 51重组修复基因家族的一个高度保守的新成员。cDNA能够显著补充独特细胞系irs 1的表型，irs 1对DNA交联剂和遗传不稳定性表现出极端敏感性。这种表型与XRCC 2基因在体细胞重组修复中的作用一致，表明除了RAD 51之外，该基因家族的其他成员在哺乳动物的高保真修复过程中具有重要功能。尽管有这种功能，XRCC 2基因转录本在体细胞组织中以非常低的水平表达，但在小鼠睾丸中升高，表明在减数分裂中的额外作用。
We recently identified a positional candidate for the XRCC2 DNA repair gene at human chromosome 7q36.1, We have now cloned the cDNA for this gene from both human and mouse and show that it is a highly conserved novel member of the recA/RAD51 recombination repair gene family. The cDNA is able to complement significantly the phenotype of a unique cell line, irs1, which shows extreme sensitivity to DNA cross-linking agents and genetic instability. This phenotype is consistent with a role for the XRCC2 gene in recombination repair in somatic cells, suggesting that in addition to RAD51, other members of this gene family have an important function in high fidelity repair processes in mammals. Despite this function, the XRCC2 gene transcript is expressed at a very low level in somatic tissue, but is elevated in mouse testis, suggesting an additional role in meiosis.