Determination of Interferon-α-Producing Capacity in Whole Blood Cultures from Patients with Various Diseases and from Healthy Persons
Determination of Interferon-α-Producing Capacity in Whole Blood Cultures from Patients with Various Diseases and from Healthy Persons
复制标题
各种疾病患者和健康人全血培养物中干扰素-α 产生能力的测定
DOI：
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发表时间：
1996
期刊：
影响因子：
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通讯作者：
T. Kishida
中科院分区：
文献类型：
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作者：
K. Uno;K. Nakano;N. Maruo;H. Onodera;H. Mata;I. Kurosu;K. Akatani;N. Ikegami;A. Kishi;Y. Yasuda;K. Tanaka;J. Setoguchi;M. Kondo;S. Muramatsu;T. Kishida
为了评估测定患者干扰素（IFN）产生能力的临床价值，我们在体外测定仙台病毒（HVJ）诱导的干扰素（IFN）在不同疾病患者全血细胞培养物中的产生。肺癌、骨髓增生异常综合征、非胰岛素依赖型糖尿病、肺结核和无症状HIV-1感染患者的IFN生成低于健康人。此外，IFN产生的周期性测量显示，随着肿瘤分期的进展，肺癌患者的IFN产生能力下降。然而，在标准治疗后的结核病患者中观察到ifn产生能力增加。进一步的实验表明，在全血培养中诱导的IFN的主要类型是IFN-α，患者体内IFN产生的减少不是由于白细胞数量的减少，而是由于细胞IFN产生的损害。评价全血中IFN的产生…
To assess the clinical value of determination of the interferon ( IFN)-producing capacity of patients, IFN production induced by Sendai virus (HVJ) in vitro was measured in cell cultures of whole blood from patients with various diseases. IFN production in patients with lung cancer, myelodysplastic syndromes, noninsulin-dependent diabetes mellitus, pulmonary tuberculosis, and asymptomatic HIV-1 infection was lower than that in healthy persons. Furthermore, periodic measurements of IFN production revealed decreasing IFN-producing capacities in patients with lung cancer with progression of the tumor stage. However, increased IFN-producing capacities were observed in patients with tuberculosis after standard therapy. Further experiments showed that the main type of IFN induced in whole blood cultures was IFN-α, and decreased IFN production in patients did not result from a decreased number of leukocytes but rather from an impairment of cellular IFN production. The evaluation of IFN production in whole blood ...