DETECTION OF HEPATITIS-B VIRUS-DNA IN HEPATOCYTES, BILE-DUCT EPITHELIUM, AND VASCULAR ELEMENTS BY INSITU HYBRIDIZATION
DETECTION OF HEPATITIS-B VIRUS-DNA IN HEPATOCYTES, BILE-DUCT EPITHELIUM, AND VASCULAR ELEMENTS BY INSITU HYBRIDIZATION
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DOI：
10.1073/pnas.80.21.6685
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发表时间：
1983-01-01
期刊：
影响因子：
--
通讯作者：
VYAS, GN
中科院分区：
文献类型：
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作者：
BLUM, HE;STOWRING, L;VYAS, GN
将特异性针对乙型肝炎病毒（HBV）核苷酸序列的放射性标记探针与 3 名慢性乙型肝炎患者的肝组织进行原位杂交。不仅在受感染的肝细胞中，而且在胆管上皮细胞、内皮细胞和平滑肌细胞中也检测到了 HBV 基因组。这些发现扩展了已知的 HBV 宿主细胞范围，提出了病毒传播的新机制，并说明了原位杂交在 HBV 感染发病机制研究中的有用性。
A radiolabeled probe specific for hepatitis B virus (HBV) nucleotide sequences was hybridized in situ to liver tissue from 3 patients with chronic hepatitis B. The HBV genome was detected not only in infected hepatocytes but also in bile duct epithelial cells, endothelial cells and smooth muscle cells. These findings extend the known host cell range for HBV, suggest new mechanisms of viral dissemination and illustrate the usefulness of in situ hybridization in the study of pathogenesis of HBV infection.