Preparation and In Vivo Evaluation of Apatite/Collagen Packed Composite by Alternate Immersion Method and Newton Press
Preparation and In Vivo Evaluation of Apatite/Collagen Packed Composite by Alternate Immersion Method and Newton Press
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DOI：
10.1002/jbm.b.31318
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发表时间：
2009-08-01
影响因子：
3.4
通讯作者：
Araki, Yoshima
中科院分区：
文献类型：
--
作者：
Taira, Masayuki;Nezu, Takashi;Araki, Yoshima
希望进一步开发生物相容的、生物可吸收的和骨传导的生物材料用于牙科和医疗诊所中的骨移植物。一种候选材料可能是高密度磷灰石/胶原复合物，它可以治愈相对较大的骨缺损。为了制备这样的复合物，我们冷冻干燥I型胶原溶液，用2wt%戊二醛交联形成的海绵，将不溶性海绵交替浸入CaCl（2）和Na（2）HPO（4）溶液中五次，并通过5000 kgf的牛顿压力压实海绵。为了比较，还压制了没有交替浸泡的交联胶原。SEM/EPMA、XRD和FTIR分析证实交替浸泡成功地用羟基磷灰石涂覆胶原海绵。将直径6 mm、高0.5mm的填充型磷灰石/胶原复合材料和胶原圆盘植入兔胫骨骨膜下2、4、8和12周，以评估生物相容性、生物可吸收性和骨传导性。组织学观察表明，填充的磷灰石/胶原复合材料具有生物相容性，骨传导性长达8周，并且在12周时基本上被生物吸收，而填充的胶原海绵引起了不期望的异物反应，随着植入期的推移而恶化。这些结果表明，这种填充的磷灰石/胶原复合材料可能被用作一种新的生物可吸收骨移植材料。(C)2009 Wiley Periodicals，Inc.生物医学材料研究杂志B部分：应用生物材料90 B：566-573，2009
Further development of bio-compatible, bio-absorbable, and osteo-conductive bio-materials is desired for bone grafts in dental and medical clinics. One candidate material might be a high-density apatite/collagen composite, which cures relatively large bone defects. To produce such a composite, we freeze-dried type I collagen solution, cross-linked the formed sponge by 2 wt % glutaraldehyde, immersed the insoluble sponge in CaCl(2) and Na(2)HPO(4) solutions alternately five times, and compacted the sponge by Newton press at 5000 kgf. For comparison, cross-linked collagen without alternate immersion was also pressed. SEM/EPMA, XRD, and FTIR analyses clarified that alternate immersion successfully coated the collagen sponge with hydroxyapatite. Packed apatite/collagen composite and collagen disks 6 mm in diameter and 0.5 mm in height were implanted in the subperiostea of rabbit tibiae for 2, 4, 8, and 12 weeks to assess bio-compatibility, bio-absorbability, and osteo-conductivity. Histological observations showed that the packed apatite/collagen composite was biocompatible, osteo-conductive for up to 8 weeks, and largely bio-absorbed at 12 weeks, while the packed collagen sponge caused an undesirable foreign body reaction, which worsened with the implantation period. The overall findings suggest that this packed apatite/collagen composite might be used as a new bio-absorbable bone graft material. (C) 2009 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 90B: 566-573, 2009