Transcriptome Comparison Reveals the Adaptive Evolution of Two Contrasting Ecotypes of Zn/Cd Hyperaccumulator Sedum alfredii Hance.
Transcriptome Comparison Reveals the Adaptive Evolution of Two Contrasting Ecotypes of Zn/Cd Hyperaccumulator Sedum alfredii Hance.
复制标题
转录组比较揭示了锌/镉超积累植物景天两种对比生态型的适应性进化
DOI：
10.3389/fpls.2017.00425
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发表时间：
2017
影响因子：
5.6
通讯作者：
Yang X
中科院分区：
文献类型：
--
作者：
Yang Q;Shohag MJI;Feng Y;He Z;Yang X
东南景天（Sedum alfredii Hance）的超积累生态型（HE）和非超积累生态型（NHE）属于同一种植物，但在镉和锌的超积累和超耐性方面表现出不同的特性。使用Illumina Hiseq 2500平台对HE和NHE进行测序，以研究该对比性状的遗传进化。获得了超过9000万个干净的读段，并基于七个现有数据库注释了118，479/228，051个HE/NHE单基因。我们确定了149，668/319，830个单核苷酸多态性（SNP）和12，691/14，428个简单序列重复（SSR）。我们利用分支位点模型对18个变异的直向同源基因和57个保守的直向同源基因进行了鉴定。东南花这些趋异基因主要参与转录和翻译过程、蛋白质代谢过程、钙离子（Ca 2+）途径、胁迫应答过程和信号转导过程。据我们所知，这是第一项使用RNA-seq比较来自同一物种的超积累和非超积累植物的遗传进化的研究。此外，本研究还为进一步利用分子标记和不同的正向基因来描述大豆超积累和超耐性性状的进化过程和形成奠定了基础。东南花
Hyperaccumulating ecotype (HE) and non-hyperaccumulating ecotype (NHE) of Sedum alfredii Hance belong to the same species but exhibit contrasting characteristics regarding hyperaccumulation and hypertolerance to cadmium and zinc. The Illumina Hiseq 2500 platform was employed to sequence HE and NHE to study the genetic evolution of this contrasting trait. Greater than 90 million clean reads were obtained and 118,479/228,051 unigenes of HE/NHE were annotated based on seven existing databases. We identified 149,668/319,830 single nucleotide polymorphisms (SNPs) and 12,691/14,428 simple sequence repeats (SSRs) of HE/NHE. We used a branch-site model to identify 18 divergent orthologous genes and 57 conserved orthologous genes of S. alfredii Hance. The divergent orthologous genes were mainly involved in the transcription and translation processes, protein metabolism process, calcium (Ca2+) pathway, stress response process and signal transduction process. To the best of our knowledge, this is the first study to use RNA-seq to compare the genetic evolution of hyperaccumulating and non-hyperaccumulating plants from the same species. In addition, this study made the sole concrete for further studies on molecular markers and divergent orthologous genes to depict the evolution process and formation of the hyperaccumulation and hypertolerance traits in S. alfredii Hance.