Subcellular localization of the amide class herbicide 3,4-dichloropropionanilide (DCPA) in T cells and hepatocytes.
Subcellular localization of the amide class herbicide 3,4-dichloropropionanilide (DCPA) in T cells and hepatocytes.
复制标题
酰胺类除草剂 3,4-二氯丙酰苯胺 (DCPA) 在 T 细胞和肝细胞中的亚细胞定位。
DOI：
10.1080/15287390903249131
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Barnett,JohnB
中科院分区：
文献类型：
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作者：
Hanson,MirandaL;Peer,CodyJ;Brundage,Rodney;Callery,PatrickS;Brundage,Kathleen;Schafer,Rosana;Eremin,Sergei;Barnett,JohnB
3,4-Dichloropropionanilide (DCPA), or propanil, a post-emergent herbicide used on rice and wheat crops in the United States, is immunotoxic in vivo and in vitro. Although it has been documented that DCPA exerts differential effects on specific immune cell types and is toxic to the liver, the way in which DCPA modulates intracellular functions leading to these effects is less understood. In this study, Jurkat T cells and hepatocytes from C57Bl/6 mice were exposed to 100 μMDCPA for 1.5 h. Following incubation, subcellular fractions of each cell type were isolated. DCPA, when present, was removed from each cell fraction by liquid–liquid extraction. The extraction product was then analyzed for the presence of DCPA using liquid chromatography–tandem mass spectrometry (LC-MS/MS). The cellular uptake of DCPA was monitored by detection of the molecular ion and product ion of DCPA. The analyses demonstrate that DCPA, a lipophilic compound, localizes primarily in the cytosol of T cells and hepatocytes. These results indicate that DCPA is able to cross the plasma membrane and is accessible to intracellular immunomodulatory effectors.
