A monoclonal immunoassay for the coplanar polychlorinated biphenyls.
A monoclonal immunoassay for the coplanar polychlorinated biphenyls.
复制标题
共面多氯联苯的单克隆免疫分析。
DOI：
10.1021/ac00117a003
复制
发表时间：
1995
影响因子：
7.4
通讯作者：
Karu,AE
中科院分区：
文献类型：
--
作者：
Chiu,YW;Carlson,RE;Marcus,KL;Karu,AE
多氯联苯（PCBs）是一种广泛存在的环境污染物，具有多种毒性、致畸性、生殖毒性、免疫毒性和致瘤性。209种多氯联苯异构体（同源物）中含量最少的三种毒性最大，最难量化。这些化合物是3，4，3 '，4'-四氯联苯、3，4，3 '，4'，5 '-五氯联苯和3，4，5，3'，4 '，5'-六氯联苯（IU-PAC编号分别为77、126和169）。设计了一种免疫半抗原，以保留这些有毒同系物的3，4，3 '，4'氯取代模式和共面性特征。免疫测定的最佳竞争者是结合力较弱的特异性单环多氯联苯片段。制备了一种单克隆抗体S2 B1，并用于直接竞争性酶免疫分析（EIA）。环境影响分析对非邻位取代同系物具有高度特异性，不能识别更普遍但毒性小得多的非共面多氯联苯同系物或2，3，7，8-四氯二苯并对二恶英、2，3，7，8-四氯二苯并呋喃或二氯苯。本试验的半抗原和竞争物设计为开发其他类别多氯联苯同系物的敏感环境影响分析奠定了基础。
Polychlorinated biphenyls (PCBs) are ubiquitous envi-ronmental pollutants with diverse toxic, teratogenic, re-productive, immunotoxic, and tumorigenic effects. Three of the least abundant of the 209 PCB isomers (congeners) are the most toxic and most difficult to quantify. These are 3, 4, 3', 4'-tetrachlorobiphenyl, 3, 4, 3', 4', 5'-pentachlo-robiphenyl, and 3, 4, 5, 3', 4', 5'-hexachlorobiphenyl (IU-PAC No. 77,126, and 169, respectively). An immunizing hapten was designed to retain the 3, 4, 3', 4'chlorinesubstitution pattern and coplanarity characteristic of these toxic congeners. The optimal competitors for immunoas-say were weaker binding distinctive single-ring fragments of the PCBs. A monoclonal antibody designated S2B1 was derived and used in direct (antibody-capture) com-petitive enzyme immunoassays (EIAs). The EIAs are highly specific for non-ortho-substituted congeners and do not recognize the more prevalent but much less toxic noncoplanar PCB congeners or 2, 3, 7, 8-tetrachloro-dibenzo-p-dioxin, 2, 3, 7, 8-tetrachlorodibenzoftiran, or dichlorobenzenes. Hapten and competitor design for this assay suggests a basis for development of sensitive EIAs for other classes of PCB congeners.