Expression profiles of EphA3 at both the RNA and protein level in the developing mammalian forebrain
Expression profiles of EphA3 at both the RNA and protein level in the developing mammalian forebrain
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DOI：
10.1002/cne.20551
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发表时间：
2005-07-04
影响因子：
2.5
通讯作者：
Nakajima, K
中科院分区：
文献类型：
--
作者：
Kudo, C;Ajioka, I;Nakajima, K
众所周知，ephrin/Eph系统调节大脑发育的各个方面。在这项研究中，我们分析了EphA3在发育中的小鼠前脑中RNA和蛋白水平的表达谱。虽然已知EphA3基因编码两种受体亚型，全长跨膜型和短分泌型，但仅在发育中的前脑中检测到全长亚型。我们发现，在发育早期，EphA3 mRNA在丘脑背侧和皮质中间带（IMZ）表达，EphA3蛋白在IMZ和内囊中检测到，而在丘脑背侧未检测到。在后期，mRNA在皮质板最浅表区表达，而蛋白在中轴区表达。mRNA和蛋白质表达模式之间的差异可能归因于蛋白质被转运到轴突以调节丘脑皮质和皮质投射的可能性。L1和EphA3或TAG-1和EphA3的双免疫染色结果表明，EphA3蛋白主要在丘脑皮质轴突产生，仅部分在皮质轴突产生。此外，EphA3蛋白在侧嗅束、前连合和胼胝体等多种结构中也被检测到，提示EphA3可能调节发育中的大脑中各种神经元网络的形成，包括TC投射和连合纤维。(c) 2005 Wiley-Liss, Inc。
The ephrin/Eph system is well known to regulate various aspects of brain development. In this study, we analyzed the expression profiles of EphA3 at both the RNA and protein level in developing mouse forebrains. Although the EphA3 gene is known to encode two isoforms of the receptors, a full-length transmembrane form, and a short, secretory form, only the full-length isoform was detected in the developing forebrain. We found that, in the early developmental stages, while EphA3 mRNA was expressed in the dorsal thalamus and the cortical intermediate zone (IMZ), the EphA3 protein was detected in the IMZ and the internal capsule, but not in the dorsal thalamus. In the later stages the mRNA was expressed in the most superficial region of the cortical plate, while the protein was expressed in the IMZ. This discrepancy between the mRNA and protein expression patterns might be attributed to the possibility of the protein being transported to the axons to regulate the thalamocortical and corticofugal projection. The results of double-immunostaining for L1 and EphA3 or TAG-1 and EphA3 suggested that EphA3 protein was produced mainly in the thalamocortical axons and only partially in the corticofugal axons. In addition, the EphA3 protein was also detected in various other structures, such as the lateral olfactory tract, anterior commissure, and corpus callosum, suggesting the possibility that EphA3 might regulate the formation of various neuronal networks in the developing brain, including the TC projection and the commissural fibers. (c) 2005 Wiley-Liss, Inc.