An integrated study on antimicrobial activity and ecotoxicity of quantum dots and quantum dots coated with the antimicrobial peptide indolicidin.
An integrated study on antimicrobial activity and ecotoxicity of quantum dots and quantum dots coated with the antimicrobial peptide indolicidin.
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DOI：
10.2147/ijn.s107752
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发表时间：
2016
影响因子：
8
通讯作者：
Falanga A
中科院分区：
文献类型：
--
作者：
Galdiero E;Siciliano A;Maselli V;Gesuele R;Guida M;Fulgione D;Galdiero S;Lombardi L;Falanga A
本研究旨在评估量子点（QDs）单独和包覆indolicidin的抗菌活性和生态毒性。为了实现这一目标，我们测试了对革兰氏阳性菌和革兰氏阴性菌的抗菌活性水平，并设计了一套生态毒理学测试系统和指标，能够使用各种终点检测不同的影响。对金黄色葡萄球菌进行抗菌活性分析（ATCC 6538），铜绿假单胞菌（ATCC 1025），大肠埃希菌（ATCC 11229）和肺炎克雷伯氏菌（ATCC 10031），并且结果显示QDs-Ind的杀菌作用改善。对大型蚤的毒性研究表明，与单独的QD相比，QDs-Ind的毒性降低，缺乏对弧菌的生物发光抑制，并且在鼠伤寒沙门氏菌TA 100中没有突变。采用彗星试验和氧化应激试验对D. magna具有遗传毒性和氧化损伤作用，并有剂量反应趋势。当与QD结合时，Indolicidin保留其活性。我们观察到量子点-吲哚的活性增强。在量子点表面的indolicidin的存在能够降低其量子点的毒性。
This study attempts to evaluate the antimicrobial activity and the ecotoxicity of quantum dots (QDs) alone and coated with indolicidin. To meet this objective, we tested the level of antimicrobial activity on Gram-positive and Gram-negative bacteria, and we designed an ecotoxicological battery of test systems and indicators able to detect different effects using a variety of end points. The antibacterial activity was analyzed against Staphylococcus aureus (ATCC 6538), Pseudomonas aeruginosa (ATCC 1025), Escherichia coli (ATCC 11229), and Klebsiella pneumoniae (ATCC 10031), and the results showed an improved germicidal action of QDs-Ind. Toxicity studies on Daphnia magna indicated a decrease in toxicity for QDs-Ind compared to QDs alone, lack of bioluminescence inhibition on Vibrio fisheri, and no mutations in Salmonella typhimurium TA 100. The comet assay and oxidative stress experiments performed on D. magna showed a genotoxic and an oxidative damage with a dose–response trend. Indolicidin retained its activity when bound to QDs. We observed an enhanced activity for QDs-Ind. The presence of indolicidin on the surface of QDs was able to decrease its QDs toxicity.