Growth and differentiation factor-9 stimulates progression of early primary but not primordial rat ovarian follicle development
Growth and differentiation factor-9 stimulates progression of early primary but not primordial rat ovarian follicle development
复制标题
DOI：
10.1095/biolreprod.101.002527
复制
发表时间：
2002-09-01
影响因子：
3.6
通讯作者：
Skinner, MK
中科院分区：
文献类型：
--
作者：
Nilsson, EE;Skinner, MK
卵巢含有大量原始卵泡，其中含有减数分裂中停滞的卵母细胞，这些卵母细胞是雌性发育卵泡的来源。生长和分化因子-9（GDF-9）是生长因子转化生长因子β超家族的成员，GDF-9敲除小鼠的卵泡停滞在发育的初级阶段。使用大鼠卵巢器官培养系统检查 GDF-9 治疗对原始卵泡向初级卵泡转变以及随后卵泡进展的影响。 4 天龄大鼠的卵巢在无血清条件下、在不存在或存在生长因子的情况下进行培养。 GDF-9处理导致1期早期初级卵泡的比例减少，同时2期成熟初级卵泡的比例增加。 GDF-9 不影响原始卵泡或 0 期到 1 期卵泡的转变。 GDF-9 也不影响第 3 或第 4 阶段的次级卵泡数量。使用分离的窦卵泡颗粒和卵泡膜细胞培养物来分析 GDF-9 的作用。 GDF-9 处理不直接影响颗粒细胞或卵泡膜细胞增殖。检查了 GDF-9 影响另一种生长因子表达的能力。培养 2 天后，GDF-9 处理增加了牛颗粒细胞中试剂盒配体 (KL) mRNA 的表达。 4 日龄大鼠的卵巢也经过或不经过 GDF-9 处理进行培养，GDF-9 增加了 KL mRNA 的总卵巢表达。总之，GDF-9被发现促进早期初级卵泡发育的进展，但不影响原始卵泡发育。 GDF-9 对卵泡发育特定阶段的作用可能部分是通过改变 KL 的表达来介导的。
(T)he ovary contains a pool of primordial follicles containing oocytes arrested in meiosis that are the source of developing follicles for the female. Growth and differentiation factor-9 (GDF-9) is a member of the transforming growth factor beta superfamily of growth factors, and follicles of GDF-9 knockout mice arrest in the primary stage of development. The effect of GDF-9 treatment on the primordial to primary follicle transition and on subsequent follicle progression was examined using a rat ovary organ culture system. Ovaries from 4-day-old rats were cultured under serum-free conditions in the absence or presence of growth factors. GDF-9 treatment caused a decrease in the proportion of stage 1 early primary follicles and a concomitant increase in the proportion of stage 2 mature primary follicles. GDF-9 did not effect primordial follicles or stage 0 to stage 1 follicle transition. GDF-9 also did not influence stage 3 or 4 secondary follicle numbers. Isolated antral follicle granulosa and theca cell cultures were used to analyze the actions of GDF-9. GDF-9 treatment did not directly influence either granulosa or theca cell proliferation. The ability of GDF-9 to influence the expression of another growth factor was examined. GDF-9 treatment increased kit ligand (KL) mRNA expression in bovine granulosa cells after 2 days of culture. Ovaries from 4-day-old rats were also cultured with or without GDF-9 treatment, and total ovary expression of KL mRNA was increased by GDF-9. In summary, GDF-9 was found to promote the progression of early primary follicle development but did not influence primordial follicle development. The actions of GDF-9 on specific stages of follicle development may in part be mediated through altering the expression of KL.