DNA Stability in Ionic Liquids and Deep Eutectic Solvents.
DNA Stability in Ionic Liquids and Deep Eutectic Solvents.
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DOI：
10.1002/jctb.4511
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发表时间：
2015-01
影响因子：
3.4
通讯作者：
Hua Zhao
中科院分区：
文献类型：
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作者：
Hua Zhao
DNA分子被称为遗传信息载体。最近，它们被探索作为新一代生物催化剂或金属催化剂的手性支架。人们越来越有兴趣寻找用于 DNA 保存和稳定的替代溶剂，包括两种独特类型的溶剂：离子液体 (IL) 和低共熔溶剂 (DES)。因此，了解 DNA 分子如何与这些新型离子溶剂系统（即 IL 和 DES）相互作用非常重要。众所周知，无机二价和单价离子优先与DNA结构的大沟和小沟结合。然而，对于 IL 和 DES，有机阳离子可能会侵入 DNA 小沟；更重要的是，有机阳离子和 DNA 磷酸主链之间的静电吸引成为主要的相互作用，同时伴随着 IL 和 DNA 大沟和小沟之间的疏水性和极性相互作用。此外，阴离子可与胞嘧啶、腺嘌呤和鸟嘌呤碱基形成氢键。尽管存在这些强烈的相互作用，DNA 分子在大多数离子溶剂系统中仍保持双螺旋结构，尤其是在 IL 水溶液中。此外，还讨论了 IL 和 DES 中 G-四链体 DNA 结构的令人兴奋的进展。
DNA molecules are known as the genetic information carriers. Recently, they are being explored as a new generation of biocatalysts or chiral scaffolds for metal catalysts. There is also a growing interest of finding alternative solvents for DNA preservation and stabilization, including two unique types of solvents: ionic liquids (ILs) and deep eutectic solvents (DES). Therefore, it is important to understand how DNA molecules interact with these novel ionic solvent systems (i.e. ILs and DES). It is well known that inorganic di- and monovalent ions preferentially bind with major and minor grooves of DNA structures. However, in the case of ILs and DES, organic cation may intrude into the DNA minor grooves; more importantly, electrostatic attraction between organic cations and the DNA phosphate backbone becomes a predominant interaction, accompanying by hydrophobic and polar interactions between ILs and DNA major and minor grooves. In addition, anions may form hydrogen-bonds with cytosine, adenine and guanine bases. Despites these strong interactions, DNA molecules maintain double helical structure in most ionic solvent systems, especially in aqueous IL solutions. Furthermore, the exciting advances of G-quadruplexe DNA structures in ILs and DES are discussed.