Tunable DNA Origami Motors Translocate Ballistically Over μm Distances at nm/s Speeds
Tunable DNA Origami Motors Translocate Ballistically Over μm Distances at nm/s Speeds
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DOI：
10.1002/anie.201916281
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发表时间：
2020-04-01
影响因子：
16.6
通讯作者：
Salaita, Khalid
中科院分区：
文献类型：
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作者：
Bazrafshan, Alisina;Meyer, Travis A.;Salaita, Khalid
受到生物运动蛋白的启发，生物运动蛋白可以有效地将化学燃料转化为单向运动，人们对开发合成类似物产生了很大的兴趣。在迄今为止创造的合成马达中，在 RNA 轨道上执行离散步骤的 DNA 马达显示出了最大的前景。尽管如此，DNA 纳米马达缺乏内在的方向性，速度低，并且在失速或解离之前执行的步骤数量有限。在此，我们报告了第一个高度可调的 DNA 折纸电机示例，该电机以 40 nm/min 的平均速度在微米距离内线性移动。重要的是，纳米电机可以单向移动，无需通过外力场或图案轨道进行干预。由于 DNA 折纸能够精确测试纳米级结构-功能关系，因此我们能够通过实验研究电机形状、底盘灵活性、腿部分布和腿部总数在调整性能中的作用。各向异性的刚性底盘与高密度的腿相结合，最大限度地提高了纳米电机的速度和耐力。
Inspired by biological motor proteins, that efficiently convert chemical fuel to unidirectional motion, there has been considerable interest in developing synthetic analogues. Among the synthetic motors created thus far, DNA motors that undertake discrete steps on RNA tracks have shown the greatest promise. Nonetheless, DNA nanomotors lack intrinsic directionality, are low speed and take a limited number of steps prior to stalling or dissociation. Herein, we report the first example of a highly tunable DNA origami motor that moves linearly over micron distances at an average speed of 40 nm/min. Importantly, nanomotors move unidirectionally without intervention through an external force field or a patterned track. Because DNA origami enables precise testing of nanoscale structure-function relationships, we were able to experimentally study the role of motor shape, chassis flexibility, leg distribution, and total number of legs in tuning performance. An anisotropic rigid chassis coupled with a high density of legs maximizes nanomotor speed and endurance.