Macrophage activity in infected areas of an experimental vertebral osteomyelitis model:: USPIO-enhanced MR imaging feasibility study
Macrophage activity in infected areas of an experimental vertebral osteomyelitis model:: USPIO-enhanced MR imaging feasibility study
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DOI：
10.1148/radiol.2481071260
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发表时间：
2008-07-01
期刊：
影响因子：
19.7
通讯作者：
Kremer, Stephane
中科院分区：
文献类型：
--
作者：
Bierry, Guillaume;Jehl, Francois;Kremer, Stephane
目的：为了前瞻性地评估超小型超顺磁性氧化铁（USPIO）磁共振（MR）成像的描述巨噬细胞在感染的实验性兔脊椎骨髓炎model.Materials和方法：腰椎骨髓炎诱导10只兔椎间盘内注射细菌在一个椎间盘（测试水平）与盐水注射在另一个磁盘（对照水平）。平均间隔12天后，在USPIO给药前和给药后24小时对家兔进行成像。磁共振成像方案包括T1加权自旋回波，T2加权快速自旋回波和T2* 加权梯度回波序列。MR表现与组织学表现（巨噬细胞免疫染色和Perls普鲁士蓝染色）进行了比较。采用Wilcoxon符号秩检验比较USPIO给药前后的信噪比（SNR）结果：USPIO给药后24小时获得的感染椎体测试水平的T1加权MR图像显示SNR显著增加（P= 0.005），而T2和T2* 加权图像显示SNR无显著变化（分别为P= 0.14和P= 0.87）。感染区的组织学检查结果显示造血骨髓被巨噬细胞浸润完全取代。Perls普鲁士蓝染色显示部分巨噬细胞负载铁。对照椎骨的T1（P= 0.02）、T2（P= 0.04）和T2* 加权（P= 0.04）图像显示SNR显著降低。组织学检查结果证实了正常造血骨髓的持久性无巨噬细胞浸润，这是反映了更强烈的珀尔斯普鲁士蓝染色相比，在感染areas.Conclusion：磁共振成像可以描述USPIO加载的巨噬细胞浸润存在于感染区的实验性兔脊椎骨髓炎模型。(c)RSNA，2008年。
Purpose: To prospectively evaluate ultrasmall superparamagnetic iron oxide (USPIO) magnetic resonance (MR) imaging for the depiction of macrophages in infected areas of an experimental rabbit vertebral osteomyelitis model.Materials and Methods: Lumbar vertebral osteomyelitis was induced in 10 rabbits with intradiscal injection of bacteria in a vertebral disk (test level) versus saline injection in another disk (control level). After a mean interval of 12 days, rabbits were imaged prior to and 24 hours after administration of USPIO. The MR imaging protocol included T1-weighted spin-echo, T2-weighted fast spin-echo, and T2*-weighted gradient-echo sequences. MR findings were compared with histologic findings (macrophage immunostaining and Perls Prussian blue staining). A Wilcoxon signed rank test was used to compare signal-to-noise ratio (SNR) results before and after USPIO administration.Results: T1-weighted MR images of infected vertebral test levels obtained 24 hours after USPIO administration showed a significant increase in SNR (P=.005), whereas T2- and T2*-weighted images showed no significant changes in SNR (P=.14 and P=.87, respectively). Histologic examination results of infected areas demonstrated complete replacement of hematopoietic bone marrow by macrophage infiltration. Perls Prussian blue staining showed that some macrophages were iron loaded. T1- (P=.02), T2- (P=.04), and T2*-weighted (P=.04) images of control vertebrae showed a significant decrease in SNR. Histologic examination results confirmed the persistence of normal hematopoietic bone marrow without macrophage infiltration, which was reflected by more intensive Perls Prussian blue staining compared with that in infected areas.Conclusion: MR imaging can depict USPIO-loaded macrophage infiltration present in infected areas in an experimental rabbit model of vertebral osteomyelitis. (c) RSNA, 2008.