Ear transplantations reveal conservation of inner ear afferent pathfinding cues
Ear transplantations reveal conservation of inner ear afferent pathfinding cues
复制标题
DOI：
10.1038/s41598-018-31952-y
复制
发表时间：
2018-09-14
影响因子：
4.6
通讯作者：
Fritzsch, Bernd
中科院分区：
文献类型：
--
作者：
Elliott, Karen L.;Fritzsch, Bernd
脊椎动物内耳神经元在目标神经元有丝分裂后，甚至在它们缺失的情况下，投射到正确的脑干核团区域。此外，青蛙移植的耳朵的传入已经被证明导航到前庭核团，这表明耳朵传入使用分子线索来寻找它们的目标。我们在鸡身上进行了异种、异种和异位移植，以研究内耳传入是否受到保守的引导分子的引导。我们发现，内耳传入可以在传入延迟后导航到前庭核团，当耳朵来自不同的物种时，小鼠。这些数据表明，引导分子在羊膜上表达了一段时间，并且是保守的。此外，我们还发现，移植到脊髓附近的鸡耳类似于后脑的背侧突出。这些结果表明，内耳传入使用保守的线索导航到正确的背腹侧脑干柱。
Vertebrate inner ear neurons project into the correct brainstem nuclei region before target neurons become postmitotic, or even in their absence. Moreover, afferents from transplanted ears in frogs have been shown to navigate to vestibular nuclei, suggesting that ear afferents use molecular cues to find their target. We performed heterochronic, xenoplastic, and heterotopic transplantations in chickens to investigate whether inner ear afferents are guided by conserved guidance molecules. We show that inner ear afferents can navigate to the vestibular nuclei following a delay in afferent entry and when the ear was from a different species, the mouse. These data suggest that guidance molecules are expressed for some time and are conserved across amniotes. In addition, we show that chicken ears transplanted adjacent to the spinal cord project dorsally like in the hindbrain. These results suggest that inner ear afferents navigate to the correct dorsoventral brainstem column using conserved cues.