Silver nanoparticles as a viricidal agent to inhibit plant-infecting viruses and disrupt their acquisition and transmission by their aphid vector
Silver nanoparticles as a viricidal agent to inhibit plant-infecting viruses and disrupt their acquisition and transmission by their aphid vector
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DOI：
10.1007/s00705-021-05280-y
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发表时间：
2021-11-05
影响因子：
2.7
通讯作者：
Farroh, Khaled Y.
中科院分区：
文献类型：
--
作者：
El Gamal, Ahmed Y.;Tohamy, Mohamed R.;Farroh, Khaled Y.
银纳米粒子（AgNPs）是一种潜在的有效的预防植物病毒性疾病的工具。本研究从植物-病毒-载体互作的角度评价银纳米颗粒对蚕豆黄花叶病毒（BYMV）的防治效果。银纳米粒子作为叶面保护剂和治疗剂进行了评价。此外，还研究了AgNPs对病毒感染及其媒介蚜虫传播的影响。结果表明，AgNPs表现出治疗杀病毒活性，并且在病毒接种后48小时施用时能够抑制BYMV。使用低至100 mg L-1的AgNP浓度防止疾病的发生，而病毒感染被完全抑制时，植物被预防性处理的AgNP在病毒接种前24小时的浓度为200 mg L-1。AgNPs被证明是高度生物反应性的，与病毒颗粒结合并抑制它们在植物组织内的复制和积累。此外，银纳米粒子，在所有测试浓度下，被发现上调病程相关基因PR-1和诱导生产的防御相关的氧化酶在处理过的植物。暴露的蚜虫AgNP处理的植物病毒收购前减少BYMV收购和传播效率的40.65%至100%，在24小时后应用程序，根据AgNP剂量。在处理后10天，在暴露于浓度为250和300 mg L-1的AgNPs后，病毒感染分别减少了36.82%和79.64%。这些结果表明，银纳米颗粒具有治疗性杀病毒活性，由于靶向病毒外壳蛋白和影响病毒载体的相互作用。因此，AgNPs可能有助于减轻田间条件下的自然疾病和病毒传播。这是第一份关于纳米材料对昆虫获得和传播植物病毒的活性的报告。
Silver nanoparticles (AgNPs) are a potentially effective tool for preventing viral plant diseases. This study was carried out to evaluate the effectiveness of AgNPs for managing bean yellow mosaic virus (BYMV) disease in faba bean plants from the plant-virus-vector interaction side. AgNPs were evaluated as foliar protective and curative agents. In addition, the effect of AgNPs on virus acquisition and transmission by its vector aphid was investigated. The results indicated that AgNPs exhibited curative viricidal activity and were able to inactivate BYMV when applied 48 hours after virus inoculation. The occurrence of disease was prevented using an AgNP concentration as low as 100 mg L-1, whereas virus infection was completely inhibited when plants were preventatively treated with AgNPs at a concentration of to 200 mg L-1 24 h before virus inoculation. AgNPs proved to be highly bio-reactive, binding to viral particles and suppressing their replication and accumulation within plant tissues. Moreover, AgNPs, at all concentrations tested, were found to upregulate the pathogenesis-related gene PR-1 and induce the production of defense-related oxidizing enzymes in treated plants. Exposure of aphids to AgNPs-treated plants before virus acquisition reduced BYMV acquisition and transmission efficiency by 40.65 to 100% at 24 h post-application, depending on the AgNP dosage. At 10 days after treatment, virus acquisition was reduced by 36.82% and 79.64% upon exposure to AgNPs at a concentration of 250 and 300 mg L-(1), respectively. These results suggest that AgNPs have curative viricidal activity due to targeting the virus coat protein and affecting virus-vector interactions. Accordingly, AgNPs may contribute to alleviating the natural disease and virus transmission under field conditions. This is the first report on the activity of nanomaterials against plant virus acquisition and transmission by insects.