Cloning of a cDNA encoding a sequence-specific single-stranded-DNA-binding protein from Rattus norvegicus.

Cloning of a cDNA encoding a sequence-specific single-stranded-DNA-binding protein from Rattus norvegicus.
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克隆编码来自褐家鼠的序列特异性单链 DNA 结合蛋白的 cDNA。

DOI:
10.1016/s0378-1119(99)00289-9
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发表时间:
1999
期刊:
影响因子:
3.5
通讯作者:
Rome,LH
Rome,LH
中科院分区:
生物学3区
文献类型:
--
作者:
Raval-Fernandes,S;Kickhoefer,VA;Rome,LH

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In this paper, we report the isolation of a cDNA clone encoding a sequence-specific single-stranded-DNA-binding protein (SSDP) from rat (Rattus norvegicus). The full-length nucleotide sequence was determined and encodes a 361 amino acid protein with a predicted molecular mass of 37.7kDa. This clone has ∼80% homology to a previously isolated partial cDNA clone for SSDP from chicken (Gallus gallus). Northern blot analysis revealed two transcripts of 2.0 and 3.0kb. The protein appears to be evolutionarily highly conserved with >97% identity between chicken, rat, mouse and human. Chicken SSDP has been proposed to be involved in the transcriptional regulation of the α2(I) collagen gene.
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