Chemical Composition and Anti-Inflammatory and Antioxidant Activities of Extracts from Cultivated Morel Mushrooms, Species of Genus Morchella (Ascomycota)
Chemical Composition and Anti-Inflammatory and Antioxidant Activities of Extracts from Cultivated Morel Mushrooms, Species of Genus Morchella (Ascomycota)
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DOI：
10.1615/intjmedmushrooms.2021039297
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发表时间：
2021-01-01
影响因子：
1.2
通讯作者：
Nair, Muraleedharan G.
中科院分区：
文献类型：
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作者：
Dissanayake, Amila A.;Mills, Gary L.;Nair, Muraleedharan G.
羊肚菌属是一种高价值的食用菌。它们因其香气、风味、肉质和健康益处而受到烹饪专家的追捧。M. rufobrunnea、红枝藓M. sextelata和M.本研究选择了美洲的药用植物，并通过体外抗炎和抗氧化试验对其药用质量进行了研究。这种取样代表了羊肚菌生产的条件（分别为室内种植、室外种植和从自然栖息地采集），用于商业市场。所有三种羊肚菌的水和甲醇提取物显示相同的色谱和生物测定的配置文件，独立于其系统发育的位置或生产方法。在抗氧化试验中，这些蘑菇的水和甲醇提取物在100 μ g/mL的抑制脂质过氧化（LPO）分别为59%-62%和33%-36%。在使用环氧合酶（考克斯-1和考克斯-2）的抗炎试验中，100 μ g/mL的水提取物和甲醇提取物显示考克斯-1酶抑制分别为53%-57%和30%-32%，考克斯-2酶抑制分别为38%-44%和16%-17%。M.的色谱纯化和光谱表征。红实木提取物提供了五种糖（化合物1 - 5）、七种有机酸（化合物6 - 13）、三种类黄酮（化合物14 - 16）、甘油三酯、游离脂肪酸和三种甾醇（化合物17 - 19）。这是纯分离物（S）-莫瑞利德（化合物6）、谷氨酸（化合物9）和油菜甾醇（化合物19）的考克斯-1和考克斯-2酶和LPO抑制活性的首次报道。该研究还显示，在25 μ g/mL时，考克斯-1（84%、33%和37%）、考克斯-2（47%、11%和22%）和LPO（74%、48%和35%）分别受到抑制。
Species of genus Morchella are high-value edible mushrooms. They are sought after by culinary experts due to their aroma, flavor, meaty texture, and health benefits. M. rufobrunnea, M. sextelata, and M. americana were chosen in this study and investigated for their medicinal quality by using in vitro anti-inflammatory and antioxidant assays. This sampling represents conditions by which morels arc produced (cultivated indoors, cultivated outdoors, and collected from natural habitats, respectively) for commercial markets. Both aqueous and methanolic extracts of all three morel species showed identical chromatographic and bioassay profiles, independent of their phylogenetic position or production method. In an antioxidant assay, aqueous and methanolic extracts of these mushrooms at 100 ag/mL inhibited lipid peroxidation (LPO) by 59%-62% and 33%-36%, respectively. In an anti-inflammatory assay using cyclooxygenase enzymes (COX-1 and COX-2), aqueous and methanolic extracts at 100 mu g/mL showed COX-1 enzyme inhibition by 53%-57% and 30%-32% and COX-2 enzyme inhibition by 38%-44% and 16%-17%, respectively. Chromatographic purification and spectroscopic characterization of M. rufobnomea extracts afforded five sugars (compounds 1-5), seven organic acids (compounds 6-13), three flavonoids (compounds 14-16), triglycerides, free fatty acids, and three sterols (compounds 17-19). This is the first report of COX-1 and COX-2 enzymes and LPO inhibitory activities of pure isolates (S)-morelid (compound 6), glutamic acid (compound 9), and brassicasterol (compound 19). This study also showed inhibitions of COX-1 (by 84%, 33%, and 37%), COX-2 (by 47%, 11%, and 22%), and LPO (by 74%, 48%, and 35%), respectively, at 25 mu g/mL.