Estrogen treatment in vivo increases keratinocyte growth factor expression in the mammary gland.
Estrogen treatment in vivo increases keratinocyte growth factor expression in the mammary gland.
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DOI：
10.1677/joe.0.1650039
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发表时间：
2000-04
期刊：
影响因子：
--
通讯作者：
Vadim Pedchenko;Walter Imagawa
中科院分区：
文献类型：
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作者：
Vadim Pedchenko;Walter Imagawa
乳腺上皮细胞的增殖和分化受全身激素和局部来源的旁分泌生长因子的联合作用调节。角质细胞生长因子（KGF）是一种潜在的候选基质因子，可能参与基质/上皮相互作用的激素控制。在这项研究中，我们研究了17 β-雌二醇（E）治疗对青春期（5周龄）和成熟（11周龄）小鼠乳腺中KGF表达的体内影响。小鼠接受皮下注射激素，之后通过乳腺RNA的核糖核酸酶保护分析来测定KGF mRNA水平。E治疗引起剂量和时间依赖性增加KGF mRNA水平在完整的小鼠从两个年龄组。无论是17 α-雌二醇或孕酮注射影响KGF mRNA水平。KGF在无实质脂肪垫和完整腺体中的相对表达的比较表明，基础和E-依赖的KGF mRNA水平不需要乳腺上皮的存在。KGF组织含量的ELISA测定表明，伴随着mRNA的上调，E治疗也增加了KGF蛋白在乳腺中的青春期前后和成熟的小鼠。这些数据表明，E治疗刺激KGF mRNA和蛋白质在体内乳腺基质中的表达，并提出了KGF在E调节乳腺发育中发挥作用的可能性。
Proliferation and differentiation of mammary epithelia are regulated by the combined action of systemic hormones and locally derived paracrine growth factors. Keratinocyte growth factor (KGF) is a potential candidate stromal factor that may participate in the hormonal control of stromal/epithelial interactions. In this study, we have examined the in vivo effect of 17beta-estradiol (E) treatment on KGF expression in mammary glands of peripubertal (5-week-old) and mature (11-week-old) mice. Mice received subcutaneous injections of hormone after which KGF mRNA levels were assayed by ribonuclease protection analysis of mammary gland RNA. E treatment caused a dose- and time-dependent increase in KGF mRNA levels in intact mice from both age groups. Neither 17alpha-estradiol nor progesterone injection affected KGF mRNA levels. Comparison of the relative expression of KGF in parenchyma-free fat pads and in intact glands demonstrated that the basal and E-dependent KGF mRNA levels did not require the presence of mammary epithelium. ELISA assay of KGF tissue content demonstrated that concomitantly with an up-regulation of mRNA, E treatment also increased KGF protein in mammary glands from peripubertal and mature mice. These data show that E treatment stimulates both KGF mRNA and protein expression in mammary stroma in vivo and raises the possibility that KGF has a role in E-regulated mammary gland development.