A real-time and in-situ monitoring of the molecular interactions between drug carrier polymers and a phospholipid membrane
A real-time and in-situ monitoring of the molecular interactions between drug carrier polymers and a phospholipid membrane
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实时原位监测药物载体聚合物和磷脂膜之间的分子相互作用。
DOI：
10.1016/j.colsurfb.2021.112161
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发表时间：
2021-10-23
影响因子：
5.8
通讯作者：
Kang, Zhenhui
中科院分区：
文献类型：
--
作者：
Ge, Yuke;Liu, Jiaojiao;Kang, Zhenhui
药物载体分子与细胞膜之间的动态相互作用在其临床应用中是不可忽视的。本文采用一种简单、无标记、无损伤的方法--光电压瞬态法，结合原子力显微镜、动态巨单层囊泡渗漏试验和细胞毒性试验，对相互作用过程进行实时监测。以聚氧乙烯（35）月桂基醚（Brij 35）和聚乙烯吡咯烷酮（PVPk 30）两种典型的聚合物分子为例，在低离子强度和中性pH条件下与磷脂双层膜相互作用。Brij 35在溶液中聚合物浓度的调节下表现出吸附-积累-透化主导的过程。相反，PVPk 30在分子的吸附-解吸和/或膜的透化-再密封之间进行动态平衡。这种差异分别解释了它们在活细胞试验中的高细胞毒性和低细胞毒性。简而言之，通过结合光电压的方法与传统的荧光显微镜方法，这项工作展示了新的想法的时间和膜的行动的聚合物表面活性剂，应考虑到他们的生物医学应用。
The dynamic interactions between drug carrier molecules and a cell membrane can not be ignored in their clinical use. Here a simple, label-free and non-invasive approach, photo-voltage transient method, combined with the atomic force microscopy, dynamic giant unilamellar vesicle leakage assay and cytotoxicity method, was employed for a real-time monitoring of the interaction process. Two representative polymer molecules, polyoxyethylene (35) lauryl ether (Brij35) and polyvinylpyrrolidone (PVPk30), were taken as examples to interact with a phospholipid bilayer membrane in a low ionic strength and neutral pH condition. Brij35 demonstrated an adsorption-accumulation-permeabilization dominated process under the modulation of polymer concentration in the solution. In contrast, PVPk30 performed a dynamic balance between adsorption-desorption of the molecules and/or permeabilization-resealing of the membrane. Such difference explains the high and low cytotoxicity of them, respectively, in the living cell tests. Briefly, through combining the photo-voltage approach with conventional fluorescent microscopy method, this work demonstrates new ideas on the time and membrane actions of polymer surfactants which should be taken into account for their biomedical applications.