Modified Pseudo-Schiff Propidium Iodide for Staining the Shoot Apical Meristem in Arabidopsis.
Modified Pseudo-Schiff Propidium Iodide for Staining the Shoot Apical Meristem in Arabidopsis.
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DOI：
10.21769/bioprotoc.4672
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发表时间：
2023-05-05
期刊：
影响因子：
0.8
通讯作者：
Ma, Ligeng
中科院分区：
文献类型：
--
作者：
Li, Ruiqi;Li, Qi;Ma, Ligeng
关键词：
使用荧光染料可视化细胞结构以表征细胞大小、形状和排列是研究组织形态和形态发生的常用方法。为了利用激光扫描共聚焦显微镜观察拟南芥茎尖分生组织（SAM），对碘化丙啶染色法进行了改进。该方法的优点主要体现在不需要传统的组织切片，直接观察SAM中边界清晰的细胞排列和典型的三层细胞。
Visualization of cell structure with fluorescent dye for characterizing cell size, shape, and arrangement is a common method to study tissue morphology and morphogenesis. In order to observe shoot apical meristem (SAM) in Arabidopsis thaliana by laser scanning confocal microscopy, we modified the pseudo-Schiff propidium iodide staining method by adding a series solution treatment to stain the deep cells. The advantage of this method is mainly reflected by the direct observation of the clearly bounded cell arrangement and the typical three-layer cells in SAM without the traditional tissue slicing.