High fat diet deviates PtC-specific B1 B cell phagocytosis in obese mice.
High fat diet deviates PtC-specific B1 B cell phagocytosis in obese mice.
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DOI：
10.1002/iid3.41
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发表时间：
2014-12
影响因子：
3.2
通讯作者：
Zhong, Xuemei
中科院分区：
文献类型：
--
作者：
Vo, Hung;Chiu, Joanna;Allaimo, Danielle;Mao, Changchuin;Wang, Yaqi;Gong, Yuefei;Ow, Hooisweng;Porter, Tyrone;Zhong, Xuemei
吞噬作用主要归因于“专业”吞噬细胞，如巨噬细胞，它们在脂肪组织炎症中起关键作用。然而，最近有报道称B1 B淋巴细胞具有巨噬细胞样吞噬活性。高脂饮食（HFD）诱导的肥胖与免疫功能障碍之间长期存在的相关性引起了我们的兴趣，我们研究了HFD如何影响B1 B细胞吞噬。大量B1 B细胞可识别磷脂酰胆碱（PtC），这是细胞膜上常见的磷脂成分。我们在这里报道，与巨噬细胞不同，B1 B细胞具有独特的ptc特异性吞噬功能。在ptc包被和非ptc对照荧光纳米颗粒存在的情况下，来自健康瘦小鼠的B1 B细胞选择性地吞噬ptc包被的微球，而来自hfd喂养的肥胖小鼠的B1 B细胞非选择性地吞噬ptc包被的微球和对照微球。在形态学上，肥胖小鼠的B1 B细胞与巨噬细胞相似，胞浆增大，吞噬更多的珠粒。我们的研究首次提示HFD可以影响B1 B细胞的吞噬，证实了HFD诱导的肥胖与免疫偏差的联系。
Phagocytosis had been attributed predominantly to “professional” phagocytes such as macrophages, which play critical roles in adipose tissue inflammation. However, recently, macrophage-like phagocytic activity has been reported in B1 B lymphocytes. Intrigued by the long-established correlation between high fat diet (HFD)-induced obesity and immune dysfunction, we investigated how HFD affects B1 B cell phagocytosis. A significant number of B1 B cells recognize phosphatidylcholine (PtC), a common phospholipid component of cell membrane. We report here that unlike macrophages, B1 B cells have a unique PtC-specific phagocytic function. In the presence of both PtC-coated and non-PtC control fluorescent nano-particles, B1 B cells from healthy lean mice selectively engulfed PtC-coated beads, whereas B1 B cells from HFD-fed obese mice non-discriminately phagocytosed both PtC-coated and control beads. Morphologically, B1 B cells from obese mice resembled macrophages, displaying enlarged cytosol and engulfed more beads. Our study suggests for the first time that HFD can affect B1 B cell phagocytosis, substantiating the link of HFD-induced obesity and immune deviation.