The effect of intravenous cyclophosphamide pulse on peripheral blood lymphocytes in lupus erythematosus patients
The effect of intravenous cyclophosphamide pulse on peripheral blood lymphocytes in lupus erythematosus patients
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DOI：
10.1007/pl00006852
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发表时间：
1997-07-01
影响因子：
4
通讯作者：
Muller, W
中科院分区：
文献类型：
--
作者：
Lacki, JK;Mackiewicz, SH;Muller, W
在本研究中，我们研究了静脉脉冲环磷酰胺（CY）对系统性红斑狼疮（SLE）患者淋巴细胞表面抗原的长期影响。使用二色和三色流式细胞术分析了 17 名红斑狼疮患者的血液样本。 CY治疗期间，T淋巴细胞（CD3+）总数减少31.4%，B淋巴细胞（CD19+）总数减少67.4%，NK细胞（CD16+）总数减少27.4%。 CY 方案结束六个月后，这些值恢复到入门水平。在研究开始时，我们观察到 CD3+ CD25+、CD3+ CD4-CD8-、CD4+ CD29+、CD19+ 和 CD19+ CD5+ 细胞的百分比增加。 CY治疗方案减少了CD3+ CD25+、CD3+ CD4-CD8-、CD19+和CD19+ CD5+细胞，但增加了CD3+ CD8+亚群。总而言之，与 CD4+ CD29+ 优势相关的 CD8+ T 细胞缺乏可能意味着免疫调节失衡，导致异常 CD4+ 细胞激活，从而导致自身免疫。 CY 治疗导致的 CD19+ 细胞减少和 CD8 细胞增加可能会降低 SLE 患者增强的免疫反应。
In the present study we investigated the longterm effect of intravenous pulse cyclophosphamide (CY) on lymphocyte surface antigens in systemic lupus erythematosus (SLE) patients. Blood samples derived from 17 lupus erythematosus patients were analysed using two-and three-colour flow cytometry. During the CY therapy, the total number of T lymphocytes (CD3+) was reduced by 31.4%, B lymphocytes (CD19+) by 67.4% and NK cells (CD16+) by 27.4%. Six months after the end of the CY regimen, these values recovered to entry levels. At the onset of the study we observed increased percentages of CD3+ CD25+, CD3+ CD4-CD8-, CD4+ CD29+, CD19+ and CD19+ CD5+ cells. The CY treatment regimen decreased the CD3+ CD25+, CD3+ CD4-CD8-, CD19+ and CD19+ CD5+ cells, but increased the CD3+ CD8+ subpopulation. Taken together, a deficiency of CD8+ T cells associated with CD4+ CD29+ predominance may imply an immune regulatory imbalance leading to abnormal CD4+ cell activation and in consequence to autoimmunity. Depletion of CD19+ cells combined with an enlargement of CD8 cells as a result of CY therapy may reduce the enhanced immune response in SLE patients.