Rhodiola crenulata extract counteracts the effect of hypobaric hypoxia in rat heart via redirection of the nitric oxide and arginase 1 pathway.
Rhodiola crenulata extract counteracts the effect of hypobaric hypoxia in rat heart via redirection of the nitric oxide and arginase 1 pathway.
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DOI：
10.1186/s12906-016-1524-z
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发表时间：
2017-01-07
影响因子：
--
通讯作者：
Lee SY
中科院分区：
文献类型：
--
作者：
Hsu SW;Chang TC;Wu YK;Lin KT;Shi LS;Lee SY
红景天根提取物（RCE）对体内缺氧的心脏保护作用最近被证实。然而，RCE产生这些影响的方式仍不清楚。本研究旨在证实RCE对急性低压缺氧暴露时心脏的保护作用，并研究其发生的机制。分别对SD大鼠进行RCE预处理和不RCE预处理，然后在模拟海拔8000 m的低压缺氧舱中暴露9 h，检测心脏精氨酸酶1 （Arg-1）和内皮型一氧化氮合酶（eNOS）的表达及相关信号通路的活性。缺氧降低了心脏eNOS磷酸化并增加了Arg-1的表达，但RCE预处理逆转了这两种反应。此外，RCE降低了缺氧诱导的氧化应激标志物活性氧（ROS）的产生、丙二醛（MDA）水平和蛋白质羰基含量。此外，RCE可以保护心肌细胞免受缺氧诱导的心脏凋亡，恢复缺氧动物AKT和p38 MAPK的磷酸化水平以及超氧化物歧化酶2 （SOD2）的含量。研究结果表明，红景天对高原疾病的作用部分是通过调节心脏eNOS和Arg-1通路介导的。本文的在线版本（doi:10.1186/s12906-016-1524-z）包含补充材料，可供授权用户使用。
Rhodiola crenulata is traditionally used as a folk medicine in Tibet for preventing high-altitude illnesses, including sudden cardiac death (SCD). The cardio-protective effects of Rhodiola crenulata root extract (RCE) against hypoxia in vivo have been recently confirmed. However, the way in which RCE produces these effects remains unclear. The present study is designed to confirm the protective effects of RCE on the heart in acute hypobaric hypoxia exposure and examine the mechanisms by which this occurs. Sprague–Dawley (SD) rats were pretreated with or without RCE and then exposed to a simulated altitude of 8000 m in a hypobaric hypoxia chamber for 9 h. The expression of cardiac arginase 1 (Arg-1) and endothelial nitric oxide synthase (eNOS) and the activity of associated signaling pathways was examined. Hypoxia reduced cardiac eNOS phosphorylation and increased Arg-1 expression, but both responses were reversed by RCE pre-treatment. In addition, RCE decreased the hypoxia-induced oxidative stress markers of reactive oxygen species (ROS) production, malondialdehyde (MDA) level, and protein carbonyl content. Furthermore, RCE protected cardiomyocytes from hypoxia-induced cardiac apoptosis and restored the phosphorylation level of AKT and p38 MAPK as well as the superoxide dismutase 2 (SOD2) content in hypoxic animals. The findings provide evidence that the effects of Rhodiola crenulata against altitude illness are partially mediated by modulation of eNOS and Arg-1 pathways in the heart. The online version of this article (doi:10.1186/s12906-016-1524-z) contains supplementary material, which is available to authorized users.
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影响因子：
64.8
作者：
Dimmeler, S;Fleming, I;Zeiher, AM
通讯作者：
Zeiher, AM
Rhodiola crenulata及其生物活性成分，盐酸和酪醇，通过抑制ROS-AMPK-PKC途径抑制缺氧引起的血浆膜相关Na，K-ATPase表达的减少。
DOI：
10.1155/2013/284150
发表时间：
2013
期刊：
Evidence-based complementary and alternative medicine : eCAM
影响因子：
--
作者：
Lee SY;Shi LS;Chu H;Li MH;Ho CW;Lai FY;Huang CY;Chang TC
通讯作者：
Chang TC
影响因子：
3.5
作者：
Lai, Mei-Chih;Lin, Jaung-Geng;Lee, Shin-Da
通讯作者：
Lee, Shin-Da
影响因子：
4.8
作者：
Nojiri, Hidetoshi;Shimizu, Takahiko;Shirasawa, Takuji
通讯作者：
Shirasawa, Takuji
影响因子：
4.8
作者：
Kaiser, RA;Bueno, OF;Molkentin, JD
通讯作者：
Molkentin, JD