Ex Vivo Electromechanical Reshaping of Costal Cartilage in the New Zealand White Rabbit Model
Ex Vivo Electromechanical Reshaping of Costal Cartilage in the New Zealand White Rabbit Model
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DOI：
10.1002/lary.23730
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发表时间：
2013-05-01
期刊：
影响因子：
2.6
通讯作者：
Wong, Brian J. F.
中科院分区：
文献类型：
--
作者：
Badran, Karam;Manuel, Cyrus;Wong, Brian J. F.
目标/假设：确定有效的机电重塑（EMR）参数的形状变化和细胞活力在离体兔肋软骨model.Study Design：离体动物研究结合计算机建模，以指导电极的位置和polarityselection.Methods：兔肋软骨固定在一个夹具，近似兔耳廓框架的形状。有限元建模用于选择初始电极几何形状、极性、间距，并估计剂量测定参数。利用猪肋软骨来完善给药参数的选择。进行参数分析，以确定电压和应用时间对组织形状变化的影响。接下来，重塑兔肋软骨，改变电压和应用时间，以确定最低参数，以产生可接受的形状变化，模仿天然耳廓软骨。使用单因素一般线性方差分析，在5点Likert量表上确定可接受的定性形状变化。共聚焦显微镜与活/死细胞活力分析确定的损伤程度和分布的活，死celles.Results：最低可接受的变形兔肋软骨被发现在4 V-3分钟。在4 V-3分钟的软骨重塑的活力分析表明，细胞损伤延伸2毫米远离每个电极与活细胞之间发现electrodes.Conclusions：4 V-3分钟的EMR参数表明适当的形状变化产生移植物，类似于本地耳廓，并包含足够的活细胞的临床评价。利用EMR建立的兔耳廓再造模型是可行的。
Objectives/Hypothesis: Determine the effective electromechanical reshaping (EMR) parameters for shape change and cell viability in the ex vivo rabbit costal cartilage model.Study Design: Ex vivo animal study combined with computer modeling to guide electrode placement and polarity selection.Methods: Rabbit costal cartilages were secured in a jig that approximated the shape of the rabbit auricle framework. Finite element modeling was used to select the initial electrode geometry, polarity, spacing, and estimate dosimetry parameters. Porcine costal cartilage was utilized to refine the selection of dosing parameters. Parametric analysis was performed to determine the effect of voltage and application time on tissue shape change. Next, rabbit rib cartilage was reshaped, varying voltage and application time to identify the lowest parameters to produce acceptable shape change mimicking native auricular cartilage. Acceptable qualitative shape change was determined on a five-point Likert scale analyzed using one-way general linear analysis of variance. Confocal microscopy with live/dead cell viability analysis determined the degree of injury and the distribution of live and dead cells.Results: The minimum acceptable deformation of rabbit costal cartilage was found at 4 V-3 minutes. Viability analysis of cartilage reshaped at 4 V-3 minutes demonstrates cell injury extending 2 mm away from each electrode with viable cells found between the electrodes.Conclusions: The EMR parameters of 4 V-3 minutes demonstrates appropriate shape change producing grafts that resemble the native auricle and contains the viable cells adequate for clinical evaluation. The rabbit auricular reconstruction model using EMR is a feasible one.