Single-molecule imaging of DNA polymerase I (Klenow fragment) activity by atomic force microscopy.
Single-molecule imaging of DNA polymerase I (Klenow fragment) activity by atomic force microscopy.
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DOI：
10.1039/c5nr06544e
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发表时间：
2016-03
期刊：
影响因子：
6.7
通讯作者：
Jie Chao;Jie Chao;P. Zhang;Qiufan Wang;Na Wu;Feng Zhang;Jun Hu;Chunhai Fan;Bin Li
中科院分区：
文献类型：
--
作者：
Jie Chao;Jie Chao;P. Zhang;Qiufan Wang;Na Wu;Feng Zhang;Jun Hu;Chunhai Fan;Bin Li
我们报告了一个DNA折纸促进单分子平台，利用原子力显微镜研究DNA复制。我们研究了大肠杆菌Klenow片段的几种功能活性。大肠杆菌DNA聚合酶I（KF）的作用，包括与模板DNA的结合、移动和解离。在完成这些作用后，形成双链DNA分子。此外，KF活性的方向被捕获，然后通过移动模板DNA上的KF结合位点来确认。
We report a DNA origami-facilitated single-molecule platform that exploits atomic force microscopy to study DNA replication. We imaged several functional activities of the Klenow fragment of E. coli DNA polymerase I (KF) including binding, moving, and dissociation from the template DNA. Upon completion of these actions, a double-stranded DNA molecule was formed. Furthermore, the direction of KF activities was captured and then confirmed by shifting the KF binding sites on the template DNA.