COMPARATIVE STUDIES OF THE FIBRINOLYTIC-ACTIVITY OF CULTURED VASCULAR CELLS
COMPARATIVE STUDIES OF THE FIBRINOLYTIC-ACTIVITY OF CULTURED VASCULAR CELLS
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DOI：
10.1016/0049-3848(79)90195-6
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发表时间：
1979-01-01
影响因子：
7.5
通讯作者：
LOSKUTOFF, DJ
中科院分区：
文献类型：
--
作者：
LEVIN, EG;LOSKUTOFF, DJ
检查培养的内皮细胞（EC）中的纤溶成分，并将其与非内皮血管细胞（NEVC）以及正常和病毒转化的成纤维细胞中的纤溶成分进行比较。所有血管细胞均表现出纤溶酶原激活剂（PA）活性。这种活性在从兔主动脉和腔静脉分离的 EC 中较低，但在从这些相同组织中分离的 NEVC 中较高，表明脉管系统的纤溶潜力并不限于内皮。低PA活性是从人脐带静脉获得的EC的特征。从牛主动脉和腔静脉分离的EC和NEVC均具有高PA活性，并且在这方面比劳斯肉瘤病毒转化的鸡胚成纤维细胞更活跃。用5次处理所有细胞。 10-8 M 佛波醇-12-肉豆蔻酸酯-13-乙酸酯是一种肿瘤促进剂，可对 PA 活性产生可变但显着的刺激。某些 EC 培养物的低 PA 活性与纤维蛋白溶解细胞抑制剂的存在相关。抑制剂活性在兔和人 EC 中较高，而在兔 NEVC 和鸡胚成纤维细胞中较低。尽管在稀疏的培养物中存在显着的抑制剂活性，但在汇合的牛主动脉 EC 的培养物中未检测到抑制剂活性。 EC 中 PA 的比活性也随着细胞密度的变化而波动，增加了 .apprx。当稀疏铺板的细胞生长至汇合时，增加 20 倍。组织相关纤溶活性代表 PA 和细胞抑制剂活性之间的平衡。 PA和抑制剂之间的关系可能随着细胞的生长状态而改变。这些活性随来源物种的不同而变化，并不一定代表任何特定的细胞类型。
The fibrinolytic components in cultured endothelial cells (EC) were examined and compared to those in non-endothelial vascular cells (NEVC) and in normal and virally-transformed fibroblasts. All vascular cells exhibited plasminogen activator (PA) activity. This activity was low in EC isolated from rabbit aorta and vena cava but high in NEVC isolated from these same tissues, indicating that the fibrinolytic potential of the vasculature is not restricted to the endothelium. Low PA activity was characteristic of EC obtained from human umbilical cord vein. Both EC and NEVC isolated from bovine aorta and vena cava had high PA activity and were even more active in this respect than chick embryo fibroblasts transformed by Rous-sarcoma virus. Treatment of all cells with 5 .times. 10-8 M phorbol-12-myristate-13-acetate, a tumor promoting agent, resulted in a variable but significant stimulation of PA activity. The low PA activity associated with some EC cultures correlated with the presence of a cellular inhibitor of fibrinolysis. Inhibitor activity was high in rabbit and human EC and low in rabbit NEVC and in chick embryo fibroblasts. No inhibitor activity was detected in cultures of confluent bovine aortic EC although there was significant inhibitor activity associated with sparsely populated cultures. The specific activity of PA in EC also fluctuated with cell density, increasing .apprx. 20-fold as sparsely plated cells grew to confluency. Tissue associated fibrinolytic activity represents a balance between the activities of PA and cellular inhibitors. The relation between PA and inhibitor may change with the growth state of the cells. These activities vary with species of origin and are not necessarily indicative of any specific cell type.