In Silico/In Vivo Insights into the Functional and Evolutionary Pathway of Pseudomonas aeruginosa Oleate-Diol Synthase. Discovery of a New Bacterial Di-Heme Cytochrome C Peroxidase Subfamily.
In Silico/In Vivo Insights into the Functional and Evolutionary Pathway of Pseudomonas aeruginosa Oleate-Diol Synthase. Discovery of a New Bacterial Di-Heme Cytochrome C Peroxidase Subfamily.
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DOI：
10.1371/journal.pone.0131462
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Manresa A
中科院分区：
文献类型：
--
作者：
Estupiñán M;Álvarez-García D;Barril X;Diaz P;Manresa A
如先前报道的，铜绿假单胞菌基因 PA2077 和 PA2078 编码 10S-DOX（10S-双加氧酶）和 7,10-DS（7,10-二醇合酶）酶，通过最近描述的油酸二醇合酶途径参与长链脂肪酸氧化。对两种酶的氨基酸序列的分析表明，每种蛋白质上存在两个血红素结合基序 (CXXCH)。系统发育分析显示这两种蛋白与细菌二血红素细胞色素 c 过氧化物酶 (Ccps) 的关系，类似于黄单胞菌。 35Y 橡胶氧化酶 RoxA。使用 RoxA (pdb 4b2n) 作为模板实现 PA2077 和 PA2078 的结构同源建模。从获得的 3D 模型中，发现预测的血红素环境中存在显着的氨基酸变化。此外，首次在铜绿假单胞菌基因组中报道了位于酶编码区中作为蛋白质进化元件的回文重复序列的存在。这些观察结果和两种蛋白质构建的系统发育树允许提出铜绿假单胞菌油酸二醇合酶操纵子的进化途径。综合获得的计算机和体内结果，我们得出结论，酶 PA2077 和 PA2078 是细菌过氧化物酶新亚家族中第一个被描述的成员，该亚家族被命名为脂肪酸二血红素细胞色素 c 过氧化物酶 (FadCcp)。
As previously reported, P. aeruginosa genes PA2077 and PA2078 code for 10S-DOX (10S-Dioxygenase) and 7,10-DS (7,10-Diol Synthase) enzymes involved in long-chain fatty acid oxygenation through the recently described oleate-diol synthase pathway. Analysis of the amino acid sequence of both enzymes revealed the presence of two heme-binding motifs (CXXCH) on each protein. Phylogenetic analysis showed the relation of both proteins to bacterial di-heme cytochrome c peroxidases (Ccps), similar to Xanthomonas sp. 35Y rubber oxidase RoxA. Structural homology modelling of PA2077 and PA2078 was achieved using RoxA (pdb 4b2n) as a template. From the 3D model obtained, presence of significant amino acid variations in the predicted heme-environment was found. Moreover, the presence of palindromic repeats located in enzyme-coding regions, acting as protein evolution elements, is reported here for the first time in P. aeruginosa genome. These observations and the constructed phylogenetic tree of the two proteins, allow the proposal of an evolutionary pathway for P. aeruginosa oleate-diol synthase operon. Taking together the in silico and in vivo results obtained we conclude that enzymes PA2077 and PA2078 are the first described members of a new subfamily of bacterial peroxidases, designated as Fatty acid-di-heme Cytochrome c peroxidases (FadCcp).
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DOI：
10.1016/j.bbalip.2014.06.010
发表时间：
2014-10-01
影响因子：
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