Ultrastructure of Cajal-like interstitial cells in the human detrusor
Ultrastructure of Cajal-like interstitial cells in the human detrusor
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DOI：
10.1007/s00441-008-0736-z
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发表时间：
2009-03-01
影响因子：
3.6
通讯作者：
Horn, Thomas
中科院分区：
文献类型：
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作者：
Rasmussen, Helle;Rumessen, Jueri J.;Horn, Thomas
本研究的目的是对人逼尿肌的Cajal(ICC)样细胞(ICC-L)进行超微结构的观察。透射电子显微镜下可见两种主要的间质细胞类型：成纤维样细胞(FLC)和成纤维细胞样细胞(FLC)。ICC-L为双极细胞，突起细长(0.04Aµm)，突起扁平，常形成分支迷路网络。可见小窝和短的膜相关致密带。胞体和胞质突起内可见线粒体、粗面内质网和高尔基体。中间丝丰富，但未见粗丝。ICC-L通过紧密对位、缝隙连接和插孔连接相互连接，但与平滑肌或神经的特殊联系不明显。FLC具有丰富的粗面内质网，但未见小凹或膜相关致密带；无缝隙连接和PSJ，中间丝稀少。免疫组织化学染色显示，CD34金标记位于肌束间与ICC-L对应的较长的胞浆突起内，而CD117金标记阴性。因此，在人类逼尿肌中存在ICC样细胞。它们是CD34免疫反应阳性的，具有肌样超微结构，明显区别于成纤维细胞样细胞。ICC-L可能类似于肠道中的Cajal间质细胞。
The aim of this ultrastructural study was to examine the human detrusor for interstitial cells of Cajal (ICC)-like cells (ICC-L) by conventional transmission electron microscopy (TEM) and immuno-transmission electron microscopy (I-TEM) with antibodies directed towards CD117 and CD34. Two main types of interstitial cells were identified by TEM: ICC-L and fibroblast-like cells (FLC). ICC-L were bipolar with slender (0.04 A mu m) flattened dendritic-like processes, frequently forming a branching labyrinth network. Caveolae and short membrane-associated dense bands were present. Mitochondria, rough endoplasmic reticulum and Golgi apparatus were observed in the cell somata and cytoplasmic processes. Intermediate filaments were abundant but no thick filaments were found. ICC-L were interconnected by close appositions, gap junctions and peg-and-socket junctions (PSJ) but no specialised contacts to smooth muscle or nerves were apparent. FLC were characterised by abundant rough endoplasmic reticulum but no caveolae or membrane-associated dense bands were observed; gap junctions and PSJ were absent and intermediate filaments were rare. By I-TEM, CD34 gold immunolabelling was present in long cytoplasmic processes corresponding to ICC-L between muscle fascicles but CD117 gold immunolabelling was negative. Thus, ICC-like cells are present in the human detrusor. They are CD34-immunoreactive and have a myoid ultrastructure clearly distinguishable from fibroblast-like cells. ICC-L may be analogous to interstitial cells of Cajal in the gut.