Genetic Diversity and Fitness of Fusarium graminearum Populations from Rice in Korea
Genetic Diversity and Fitness of Fusarium graminearum Populations from Rice in Korea
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DOI：
10.1128/aem.02287-08
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发表时间：
2009-05-15
影响因子：
4.4
通讯作者：
Lee, Yin-Won
中科院分区：
文献类型：
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作者：
Lee, Jungkwan;Chang, In-Young;Lee, Yin-Won
禾谷镰刀菌（Fusarium graminearum）是禾谷类作物的重要真菌病原菌，并产生真菌毒素，如雪腐镰刀菌烯醇和脱氧雪腐镰刀菌烯醇。这个物种可以被细分为一系列的遗传谱系或系统发育的物种。我们鉴定了F.从大韩民国的禾谷镰刀菌中分离出10个菌株，检测其产生雪腐镰刀菌烯醇和脱氧雪腐镰刀菌烯醇的能力，并确定其回收群体的遗传组成和结构。基于扩增片段长度多态性（AFLP），PCR基因分型，和化学分析的单孢菌素，所有249个菌株来自南方省份属于谱系6，241具有雪腐镰刀菌烯醇基因型和8具有脱氧雪腐镰刀菌烯醇基因型。在韩国东部省，我们回收了84个谱系6菌株与雪腐镰刀菌烯醇基因型和23个谱系7菌株与脱氧雪腐镰刀菌烯醇基因型。在333个6系分离物中，多态性条带占36%，有270个多位点单倍型。居群间遗传一致性较高（> 0.972），基因型多样性较低（30 ~ 58%）。为了测试谱系6对稻的适应性，将来自谱系3、6和7的菌株的分生孢子混合物接种到稻植物上，然后从产生的稻粒中回收。代表谱系6和7的菌株以远高于代表谱系3的菌株的相似频率从接种的小穗中回收。在水稻秸秆上产生大量的子囊菌，从247个子囊菌中分离到247个单子囊孢子菌株。代表谱系6的外皮（87%）最常见，其次是代表谱系7的外皮（13%），未检测到代表谱系3的外皮。这些结果表明F. graminearum谱系6可能对水稻具有寄主偏好，并且它可能比韩国存在的其他谱系更适合于水稻农业生态系统。
Fusarium graminearum is an important fungal pathogen of cereal crops and produces mycotoxins, such as the trichothecenes nivalenol and deoxynivalenol. This species may be subdivided into a series of genetic lineages or phylogenetic species. We identified strains of F. graminearum from the Republic of Korea to lineage, tested their ability to produce nivalenol and deoxynivalenol, and determined the genetic composition and structure of the populations from which they were recovered. Based on amplified fragment length polymorphism (AFLP), PCR genotyping, and chemical analyses of trichothecenes, all 249 isolates from southern provinces belonged to lineage 6, with 241 having the nivalenol genotype and 8 having the deoxynivalenol genotype. In the eastern Korea province, we recovered 84 lineage 6 isolates with the nivalenol genotype and 23 lineage 7 isolates with the deoxynivalenol genotype. Among 333 lineage 6 isolates, 36% of the AFLP bands were polymorphic, and there were 270 multilocus haplotypes. Genetic identity among populations was high (> 0.972), and genotype diversity was low (30 to 58%). To test the adaptation of lineage 6 to rice, conidial mixtures of strains from lineages 3, 6, and 7 were inoculated onto rice plants and then recovered from the rice grains produced. Strains representing lineages 6 and 7 were recovered from inoculated spikelets at similar frequencies that were much higher than those for the strain representing lineage 3. Abundant perithecia were produced on rice straw, and 247 single-ascospore isolates were recovered from 247 perithecia. Perithecia representing lineage 6 (87%) were the most common, followed by those representing lineage 7 (13%), with perithecia representing lineage 3 not detected. These results suggest that F. graminearum lineage 6 may have a host preference for rice and that it may be more fit in a rice agroecosystem than are the other lineages present in Korea.