Combined action of the major secreted exo- and endopolygalacturonases is required for full virulence of Fusarium oxysporum
Combined action of the major secreted exo- and endopolygalacturonases is required for full virulence of Fusarium oxysporum
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DOI：
10.1111/mpp.12283
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发表时间：
2016-04-01
影响因子：
4.9
通讯作者：
Roncero, M. Isabel G.
中科院分区：
文献类型：
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作者：
Bravo Ruiz, Gustavo;Di Pietro, Antonio;Roncero, M. Isabel G.
番茄病原体尖孢镰刀菌 (Fusarium oxysporum) 的基因组。 sp。 lycopersici 编码八种不同的多聚半乳糖醛酸酶 (PG)：四种 endoPG 和四种 exoPG。定量实时逆转录聚合酶链反应（RT-PCR）显示，在柑橘果胶、聚半乳糖醛酸或单体半乳糖醛酸的生长过程中以及在番茄植物的感染过程中，endoPGs pg1和pg5以及exoPGs pgx4和pgx6以显着水平表达。其余 PG 基因在所有测试条件下均表现出低表达水平。在缺少 pg1 或 pgx6 的单缺失突变体以及双突变体中，在果胶上生长期间，分泌的 PG 活性显着降低。虽然单缺失突变体对番茄植株的毒力没有显着降低，但Delta pg1 Delta pgx6双突变体的毒力显着减弱。因此，exoPGs 和 endoPGs 的联合作用对于维管束枯萎真菌尖孢镰刀菌 (F. oxysporum) 的植物感染至关重要。
The genome of the tomato pathogen Fusarium oxysporum f. sp. lycopersici encodes eight different polygalacturonases (PGs): four endoPGs and four exoPGs. Quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR) revealed that endoPGs pg1 and pg5 and exoPGs pgx4 and pgx6 are expressed at significant levels during growth on citrus pectin, polygalacturonic acid or the monomer galacturonic acid, as well as during the infection of tomato plants. The remaining PG genes exhibit low expression levels under all the conditions tested. Secreted PG activity was decreased significantly during growth on pectin in the single deletion mutants lacking either pg1 or pgx6, as well as in the double mutant. Although the single deletion mutants did not display a significant virulence reduction on tomato plants, the Delta pg1 Delta pgx6 double mutant was significantly attenuated in virulence. The combined action of exoPGs and endoPGs is thus essential for plant infection by the vascular wilt fungus F. oxysporum.