Optimization of excitation-emission band-pass filter for visualization of viable bacteria distribution on the surface of pork meat
Optimization of excitation-emission band-pass filter for visualization of viable bacteria distribution on the surface of pork meat
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DOI:
10.1364/oe.21.012579
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发表时间:
2013-05-20
期刊:
影响因子:
3.8
通讯作者:
Nakauchi, Shigeki
中科院分区:
文献类型:
--
作者:
Nishino, Ken;Nakamura, Kazuaki;Nakauchi, Shigeki
A novel method of optically reducing the dimensionality of an excitation-emission matrix (EEM) by optimizing the excitation and emission band-pass filters was proposed and applied to the visualization of viable bacteria on pork. Filters were designed theoretically using an EEM data set for evaluating colony-forming units on pork samples assuming signal-to-noise ratios of 100, 316, or 1000. These filters were evaluated using newly measured EEM images. The filters designed for S/N = 100 performed the best and allowed the visualization of viable bacteria distributions. The proposed method is expected to be a breakthrough in the application of EEM imaging. (C) 2013 Optical Society of America