Epidural Fat Tissue Is More Effective for Scar Prevention Than Conventional Subcutaneous Fat Grafting After Laminectomy in a Mouse Model
Epidural Fat Tissue Is More Effective for Scar Prevention Than Conventional Subcutaneous Fat Grafting After Laminectomy in a Mouse Model
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DOI：
10.1097/brs.0000000000004281
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发表时间：
2021-11
期刊：
影响因子：
3
通讯作者：
Kazuhiro Hata;Kazu Kobayakawa;H. Saiwai;Tetsuya Tamaru;Hirotaka Lura;Yohei Haruta;Gentarou Ono;Kazuki Kitade;T. Maeda;Y. Nakashima;Seiji Okada
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作者：
Kazuhiro Hata;Kazu Kobayakawa;H. Saiwai;Tetsuya Tamaru;Hirotaka Lura;Yohei Haruta;Gentarou Ono;Kazuki Kitade;T. Maeda;Y. Nakashima;Seiji Okada
在小鼠和人类中，EFT 与 SFT 具有不同的分子和组织学特征。在小鼠模型中，EFT 移植比 SFT 移植更有效地抑制硬膜外粘连。这些结果表明，在临床实践中，EFT 移植比传统的 SFT 移植更能有效地预防硬膜外粘连。研究设计。基础科学研究。客观的。本研究的目的是探讨硬膜外脂肪组织（EFT）移植是否比皮下脂肪组织（SFT）移植更有效地预防椎板切除术后硬膜外粘连。背景数据摘要。椎板切除术后硬膜外粘连几乎是不可避免的。尽管已经使用了许多材料来防止粘连，但没有一种材料被广泛接受。由于EFT是位于硬脑膜上的异位脂肪组织，且EFT与硬脑膜之间不存在粘连，因此我们重点关注EFT预防粘连的功效。方法。我们检查了小鼠 EFT 和 SFT 的组织学和基因表达差异。我们在第 10 胸椎进行了椎板切除术，并立即将 EFT 或 SFT 移植到小鼠的硬脑膜上。移植后6周，我们进行组织学和基因表达分析并评估粘附强度。此外，我们还研究了人类 EFT 和 SFT 之间的特征差异。结果。在小鼠和人类中，EFT 的脂肪细胞明显小于 SFT 的脂肪细胞。 SFT中炎症细胞因子和纤维化相关因子的基因表达量显着高于EFT。移植后6周时，EFT组硬脑膜上剩余脂肪面积百分比显着高于SFT组，且EFT组粘附强度评分显着低于SFT组。 RNA测序分析揭示了人类EFT和SFT之间存在1921个差异表达基因（DEG），并且与炎症反应相关的基因本体术语在SFT中最为丰富。结论。 EFT 与 SFT 具有不同的分子和组织学特征，在小鼠模型中，EFT 移植比传统的 SFT 移植在预防硬膜外粘连方面更有效。证据级别：不适用
EFT has different molecular and histological profiles from SFT in both mice and humans. In a mouse model, EFT transplantation suppressed epidural adhesion more efficiently than SFT transplantation. These results suggest that EFT transplantation would be more effective for preventing epidural adhesion than conventional SFT transplantation in clinical practice. Study Design. Basic science study. Objective. The aim of this study was to examine whether epidural fat tissue (EFT) transplantation can prevent epidural adhesion after laminectomy more efficiently than subcutaneous fat tissue (SFT) transplantation. Summary of Background Data. Epidural adhesion is almost inevitable after laminectomy. Although many materials have been used to prevent adhesion, none has been widely accepted. As EFT is an ectopic fat tissue located on the dura mater and there is no adhesion between EFT and the dura mater, we focused on the efficacy of EFT for adhesion prevention. Methods. We examined the differences in histology and gene expression between EFT and SFT of mice. We performed laminectomy at the 10th thoracic level and immediately transplanted EFT or SFT to the dura mater in mice. At 6 weeks after transplantation, we performed histological and gene expression analyses and evaluated the adhesion tenacity. In addition, we examined the characteristic differences between human EFT and SFT. Results. The adipocytes of EFT were significantly smaller than those of SFT in mice and humans. The gene expression of inflammatory cytokine and fibrosis-related factors was significantly higher in SFT than in EFT. At 6 weeks after transplantation, the percentage of the remaining fat area over the dura mater was significantly greater in the EFT group than in SFT group, and the adhesion tenacity score was significantly lower in the EFT group than that in the SFT group. An RNA sequencing analysis revealed 1921 differentially expressed genes (DEGs) between human EFT and SFT, and a Gene Ontology term associated with the inflammatory response was most highly enriched in SFT. Conclusion. EFT has different molecular and histological profiles from SFT and EFT grafting is more effective for epidural adhesion prevention than conventional SFT transplantation after laminectomy in a mouse model. Level of Evidence: N/A