Methods for the genetics and molecular biology of Candida albicans.
Methods for the genetics and molecular biology of Candida albicans.
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白色念珠菌的遗传学和分子生物学方法。
DOI：
10.1016/0003-2697(88)90559-3
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发表时间：
1988
影响因子：
2.9
通讯作者：
Magee,BB
中科院分区：
文献类型：
--
作者：
Magee,PT;Rikkerink,EH;Magee,BB
可能被认为是纯双性白色念珠菌的机会性二态真菌康-营养菌一直是研究野生型等位基因的重要合子，部分原因是它在营养缺陷菌中发生突变，在免疫功能低下患者的营养缺陷菌有限谱中不再产生越来越重要的致病作用。区域调查-辐射，二倍体门选范围的另一个预测，从粘附到各种假设，因为有丝分裂杂交产生酵母和菌丝形式的细胞类型到互惠产物（7,8）。分泌酶在发病机制中的重组作用（携带缺陷营养因子3的逆转物的l-分析）。对连锁突变进行的大量研究也支持了二态转变所涉及的因素，即这些回基因是杂合的(1)。最近的表型报告，因此，二倍体(9)。它支持了这种有机体的能力，导致了集落型的观点，即辐射诱导有丝分裂交叉和形态转变发生在1 / 2，正如每次细胞分裂的10d2到10e4的交叉频率所预测的那样，在假设中，重组有时使生物体更有趣，从生物学的角度来看，只有远端基因达到纯合性，可能会重新，而在其他时候，它将两者都与其发病机制联系在一起（4-6）。多年来，突变变成了纯合性。对这种生物的生物化学鉴定工作受到比较信息的实验的阻碍，因为它是真菌不完美的成员，缺乏任何方法来接近基因组的遗传学，内容和它的物理大小，进一步支持二倍体的解释，并且没有已知的性循环。(10)。1980年，惠兰等人（7,5）在尝试用紫外线照射制造营养不良细胞时发现，该生物体是二倍体。一种特定菌株产生一种或几种营养缺陷的趋势证明了这一点。有人认为这是由于先前存在的杂合子在辐照下通过有丝分裂交叉而变成纯合子。这一解释得到了证明的支持，即如果营养不良菌株通过选择复合体而成为杂合的，那么在进一步的照射下，同样的营养不良标记会重新出现。相反，原始细胞的原生姐妹细胞（孪生点）
The opportunistic dimorphic fungus Cun-trophs, which might be expected to be homodida albicans has been the subject of a great zygous for the wild-type allele of the gene deal of investigation, due in part to its in-mutated in the auxotroph, no longer yielded creasing importance in causing disease in im-a restricted spectrum of auxotrophs upon irmunocompromised patients. Areas investi-radiation, another prediction of the diploid gated range from the adherence to various hypothesis since mitotic crossing-over yields cell types of the yeast and hyphal forms to the reciprocal products (7, 8). Recombination role of secreted enzymes in pathogenesis (l-analysis of revertants of auxotrophs carrying 3). Extensive studies have been carried out on linked mutations also supported the hypothethe factors involved in the dimorphic transi-sis that such revertants were heterozygous tion (1). Recent reports of phenotypic insta-and, therefore, diploid (9). It supported the bility in this organism, leading to colony-type idea that irradiation induces mitotic crossingand morphological transitions which occur at over since, as predicted by the crossingfrequencies of 10d2 to 10e4 per cell division, over hypothesis, recombination sometimes make the organism even more interesting brought only the distal gene to homozygosity, from a biological point of view and may re-whereas at other times it brought both linked late to its pathogenesis (4-6). For many years mutations to homozygosity. Biochemical exthe work on this organism was hampered by periments comparing the information conthe lack of any way to approach the genetics, tent of the genome with its physical size since it is a member of the Fungi Imperfecti further supported the diploid interpretaand has no known sexual cycle. tion (10).In 1980, Whelan et al.(7, s) found, in trying to make auxotrophs using ultraviolet irradiation, that the organism was diploid. This was evidenced by the tendency for a particular strain to yield one or a few kinds of auxotrophs. It was suggested that this was due to preexisting heterozygosities which were rendered homozygous by mitotic crossing-over upon irradiation. This interpretation was supported by the demonstration that if the auxotrophic strains were rendered heterozygous by selection of revertants, the same auxotrophic markers reappeared upon further irradiation. By contrast, prototrophic sister cells (twin spots) of the original auxo-