TRIM5α cytoplasmic bodies are highly dynamic structures
TRIM5α cytoplasmic bodies are highly dynamic structures
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DOI：
10.1091/mbc.e06-12-1075
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发表时间：
2007-06-01
影响因子：
3.3
通讯作者：
Hope, Thomas J.
中科院分区：
文献类型：
--
作者：
Campbell, Edward M.;Dodding, Mark P.;Hope, Thomas J.
三联基序（TRIM）5α最近被鉴定为一种宿主限制因子，它能够以物种依赖的方式阻断某些逆转录病毒的感染。这种蛋白质的一个有趣特征是它定位于被称为胞质体的不同胞质簇中。这些胞质体在TRIM5α功能中的潜在作用仍有待确定。通过使用荧光融合蛋白和活细胞显微镜技术，我们研究了TRIM5α胞质体的定位和动态变化。该分析显示胞质体具有高度的移动性，既表现出短距离的跳跃运动，又表现出沿微管网络的单向长距离运动。胞质体的形态也是动态的。最后，光漂白和光活化分析表明，存在于胞质体中的TRIM5α蛋白是非常动态的，在胞质体和更弥散的胞质群体之间快速交换。因此，TRIM5α胞质体是动态结构，更符合在功能或调节中的作用，而不是代表无活性静态结构的蛋白质聚集体或包涵体。
Tripartite motif (TRIM)5 alpha has recently been identified as a host restriction factor that has the ability to block infection by certain retroviruses in a species-dependent manner. One interesting feature of this protein is that it is localized in distinct cytoplasmic clusters designated as cytoplasmic bodies. The potential role of these cytoplasmic bodies in TRIM5 alpha function remains to be defined. By using fluorescent fusion proteins and live cell microscopy, we studied the localization and dynamics of TRIM5 alpha cytoplasmic bodies. This analysis reveals that cytoplasmic bodies are highly mobile, exhibiting both short saltatory movements and unidirectional long-distance movements along the microtubule network. The morphology of the cytoplasmic bodies is also dynamic. Finally, photobleaching and photoactivation analysis reveals that the TRIM5 alpha protein present in the cytoplasmic bodies is very dynamic, rapidly exchanging between cytoplasmic bodies and a more diffuse cytoplasmic population. Therefore, TRIM5 alpha cytoplasmic bodies are dynamic structures more consistent with a role in function or regulation rather than protein aggregates or inclusion bodies that represent dead-end static structures.