A Hyaluronic Acid Based Injectable Hydrogel Formed via Photo-Crosslinking Reaction and Thermal-Induced Diels-Alder Reaction for Cartilage Tissue Engineering
A Hyaluronic Acid Based Injectable Hydrogel Formed via Photo-Crosslinking Reaction and Thermal-Induced Diels-Alder Reaction for Cartilage Tissue Engineering
复制标题
通过光交联反应和热诱导狄尔斯-阿尔德反应形成的基于透明质酸的可注射水凝胶，用于软骨组织工程
DOI：
10.3390/polym10090949
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发表时间：
2018-09-01
期刊：
影响因子：
5
通讯作者：
Chen, Xiaofeng
中科院分区：
文献类型：
--
作者：
Wang, Gang;Cao, Xiaodong;Chen, Xiaofeng
通过光交联反应和热诱导Diels-Alder （DA）反应合成了一种力学性能逐渐提高的透明质酸（HA）基注射水凝胶。该可注射水凝胶在苯基-2,4,6-三甲基苯甲酰膦酸锂（LAP）的催化下，通过ha -呋喃的光交联，在30 s内快速凝胶化。这种可注射性有利于保持被包膜细胞的活性，便于临床操作。力学性能可通过暴露时间控制在4.86 ~ 10.66 kPa范围内。然后，呋喃基团与马来酰亚胺基团之间进一步发生热诱导的DA咔嗒化学反应，逐渐提高了可注射水凝胶的交联密度。可注射水凝胶的力学性能可提高到21 kPa。ATDC-5细胞被成功包裹在可注射的水凝胶中，并表现出良好的活性。结果表明，通过光交联反应和热诱导DA反应形成的力学性能逐渐提高的可注射水凝胶在软骨组织工程中具有良好的应用前景。
A hyaluronic acid (HA) based injectable hydrogel with gradually increasing mechanical properties was synthesized via photo-crosslinking reaction and thermal-induced Diels-Alder (DA) reaction. The injectable hydrogel can quickly gelate within 30 s by photo-crosslinking of HA-furan under the catalysis of lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP). This injectable property is beneficial to keep the encapsulated cell activity and convenient for clinical operation. And the mechanical properties can be control from 4.86 to 10.66 kPa by exposure time. Then, the thermal-induced DA click chemistry further occurs between furan groups and maleimide groups which gradually promoted the crosslinking density of the injectable hydrogel. The mechanical properties of the injectable hydrogel can be promoted to 21 kPa. ATDC-5 cells were successfully encapsulated in the injectable hydrogel and showed good activity. All the results suggested that the injectable hydrogel with gradually increasing mechanical properties formed by photo-crosslinking reaction and thermal-induced DA reaction has a good prospect of application in cartilage tissue engineering.