The use of the polymerase chain reaction (PCR) to detect Mycobacterium tuberculosis in ancient skeletons
The use of the polymerase chain reaction (PCR) to detect Mycobacterium tuberculosis in ancient skeletons
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使用聚合酶链反应（PCR）检测古代骨骼中的结核分枝杆菌
DOI：
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发表时间：
1993
期刊：
影响因子：
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通讯作者：
E. Lemma
中科院分区：
文献类型：
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作者：
M. Spigelman;E. Lemma
聚合酶链式反应已被用于从各种不同类型的材料中提取古代DNA。我们已经考虑了使用该技术在可能已感染结核分枝杆菌的骨中发现残留细菌DNA的可能性。我们提出了一种方法，收集样本和测试的存在下降解的遗传物质在古代骨骼。聚合酶链反应的步骤进行了详细的讨论，因为是那些用于制备的骨样品。结果表明，该技术可以为我们提供有关古代疾病的新信息，在骨考古学研究中有广泛的应用。对古骨中细菌DNA的研究提出了展望。
The polymerase chain reaction has been used to extract ancient DNA from a wide range of different types of material. We have considered the possibility of finding residual bacterial DNA in bone that may have been infected with Mycobacterium tuberculosis using this technique. We propose a method of collecting samples and testing for the presence of degraded genetic material in ancient bone. The steps of the polymerase chain reaction are detailed and discussed, as are those for the preparation of the bone sample. Results obtained would suggest that this technique could have wide application in osteoarchaeological research by giving us new information on diseases of antiquity. Future avenues for the investigation of bacterial DNA in ancient bone are suggested.