The transcription activator steroidogenic factor-1 is preferentially expressed in the human pituitary gonadotroph
The transcription activator steroidogenic factor-1 is preferentially expressed in the human pituitary gonadotroph
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DOI：
10.1210/jc.81.6.2165
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发表时间：
1996-06-01
影响因子：
5.8
通讯作者：
Ezzat, S
中科院分区：
文献类型：
--
作者：
Asa, SL;Bamberger, AM;Ezzat, S
甾体生成因子-1 (SF-1)，又称肾上腺素-4结合蛋白，是一种转录因子；对类固醇生成组织的分化很重要。我们研究了SE-1是否在人类垂体及其腺瘤的特定激素产生细胞类型中表达。手术时收集垂体腺瘤（n = 35），尸检获得正常腺垂体；3例皮质促性腺瘤被排除，因为pit-1信使核糖核酸（mRNA）表达表明受到非肿瘤元素的污染。逆转录- pcr检测SF-1 mRNA的表达。用免疫细胞化学方法定位SF-1蛋白。通过逆转录pcr检测，SE-1 mRNA在垂体腺瘤和非瘤垂体中表达促性腺激素。8例促性腺腺瘤均有强烈的SF-1信号。SE-1 mRNA在3例皮质性腺瘤中的2例、13例生长/乳腺生长营养腺瘤中的2例、6例乳营养腺瘤中的1例和2例沉默型3亚型腺瘤中也检测到；然而，在大多数阳性肿瘤中，FSH β和/或LH β mRNA也呈阳性，提示污染非肿瘤性腺激素可能是SF-1 mRNA信号的来源。通过免疫细胞化学方法，SF-1蛋白被定位于双免疫染色显示为促性腺激素的非肿瘤腺垂体和促性腺激素腺瘤的分散细胞的细胞核中；在其他类型的腺瘤中未发现核染色，除了显示包含被困的非肿瘤组织的区域。我们得出结论，SF-1的表达与促性腺激素的表达相关。这些发现暗示sf - 1是一种细胞特异性转录因子，可能调节垂体促性腺激素的分化。
Steroidogenic factor-1 (SF-1), also known as adrenal-4-binding protein, is a transcription factor thai; is important for the differentiation of steroidogenic tissues. We investigated whether SE-1 is expressed in specific hormone-producing cell types in the human pituitary and its adenomas. Pituitary adenomas (n = 35) were collected at the time of surgery, and normal adenohypophyses were obtained from autopsies; 3 corticotroph adenomas were excluded because pit-1 messenger ribonucleic acid (mRNA) expression indicated contamination by nontumorous elements. Expression of SF-1 mRNA was determined by reverse transcription-PCR. SF-1 protein was localized with immuno-cytochemistry. By reverse transcription-PCR, SE-1 mRNA was found in the nontumorous pituitary and in pituitary adenomas expressing gonadotropins. All 8 gonadotroph adenomas had a strong signal for SF-1. SE-1 mRNA was also detected in 2 of 3 corticotroph adenomas, 2 of 13 somatotroph/mammosomatotroph adenomas, 1 of 6 lactotroph adenomas, and 2 silent subtype 3 adenomas; however, in most of the positive tumors there was also positivity for FSH beta and/or LH beta mRNA, suggesting that contaminating nontumorous gonadotrophs may be the source of SF-1 mRNA signal. SF-1 protein was localized by immunocytochemistry in the nuclei of scattered cells of the nontumorous adenohypophysis that were shown to be gonadotrophs with double immunostaining and in gonadotroph adenomas; nuclear staining was not found in other adenoma types, except in areas shown to contain trapped nontumorous tissue.We conclude that SF-1 expression correlates with the expression of gonadotropins. These findings implicate SF-l as a cell-specific transcription factor that may regulate gonadotroph differentiation in the pituitary.