The influence of the structure of the Au(110) surface on the ordering of a monolayer of cytochrome P450 reductase at the Au(110)/phosphate buffer interface.
The influence of the structure of the Au(110) surface on the ordering of a monolayer of cytochrome P450 reductase at the Au(110)/phosphate buffer interface.
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DOI：
10.1002/pssb.201350063
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发表时间：
2014-03-01
影响因子：
1.6
通讯作者：
Weightman, P.
中科院分区：
文献类型：
--
作者：
Smith, C. I.;Convery, J. H.;Khara, B.;Scrutton, N. S.;Weightman, P.
在−为0.652和0.056 V时，从Au(110)/磷酸盐缓冲液界面观察到的反射各向异性谱(RAS)分别与Au(110)(1×3)和阴离子诱导(1×1)有序表面结构的反射各向异性谱非常相似。在72小时内循环这两个值之间的电势后，这些RAS轮廓转变为共同的轮廓，表明形成了较不有序的表面。人黄素蛋白细胞色素P450还原酶的P499C变异体在0.056 V处吸附在有序的Au(110)/磷酸盐缓冲液界面上的单分子膜的RAS表明，来自有序的层，其中光学偶极跃迁位于大致垂直于表面并平行于Au(110)表面的[11̄0]或[001]轴的平面上。在−0.652 V时，P499C在有序界面上的吸附也得到了相同的结果。P499C在无序表面上的吸附没有导致有序单分子层的形成，证实了分子有序性受到Au(110)表面的局部结构和长程宏观有序的强烈影响。
The reflection anisotropy spectra (RAS) observed initially from Au(110)/phosphate buffer interfaces at applied potentials of −0.652 and 0.056 V are very similar to the spectra observed from ordered Au(110) (1 × 3) and anion induced (1 × 1) surface structures respectively. These RAS profiles transform to a common profile after cycling the potential between these two values over 72 h indicating the formation of a less ordered surface. The RAS of a monolayer of a P499C variant of the human flavoprotein cytochrome P450 reductase adsorbed at 0.056 V at an ordered Au(110)/phosphate buffer interface is shown to arise from an ordered layer in which the optical dipole transitions are in a plane that is orientated roughly normal to the surface and parallel to either the [11̄0] or [001] axes of the Au(110) surface. The same result was found previously for adsorption of P499C on an ordered interface at −0.652 V. The adsorption of P499C at the disordered surface does not result in the formation of an ordered monolayer confirming that the molecular ordering is strongly influenced by both the local structure and the long range macroscopic order of the Au(110) surface.
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DOI：
10.1209/0295-5075/83/18004
发表时间：
2008-07
期刊：
Europhysics letters
影响因子：
--
作者：
Messiha HL;Smith CI;Scrutton NS;Weightman P
通讯作者：
Weightman P
影响因子：
1.9
作者：
della Rocca, B. Morozzo;Smith, C. I.;Weightman, P.
通讯作者：
Weightman, P.
影响因子：
2.4
作者：
Convery, J. H.;Smith, C. I.;Weightman, P.
通讯作者：
Weightman, P.
影响因子：
1.9
作者：
Martin, DS;Blanchard, NP;Weightman, P
通讯作者：
Weightman, P
影响因子：
3.3
作者：
Salim, M.;Siddiqui, U.;Stanley, Robert J.
通讯作者：
Stanley, Robert J.