Bioorthogonal Reactions Amplify Magnetic Nanoparticles Binding and Assembly for Ultrasensitive Magnetic Resonance Sensing
Bioorthogonal Reactions Amplify Magnetic Nanoparticles Binding and Assembly for Ultrasensitive Magnetic Resonance Sensing
复制标题
生物正交反应放大磁性纳米颗粒的结合和组装，实现超灵敏磁共振传感
DOI：
10.1021/acs.analchem.9b05097
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发表时间：
2020-02-04
影响因子：
7.4
通讯作者：
Chen, Yiping
中科院分区：
文献类型：
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作者：
Zheng, Wenshu;Zeng, Lingwen;Chen, Yiping
传统的横向弛豫时间（T-2）介导的磁共振传感器（MRS），利用目标诱导的磁性纳米粒子（MNP）的状态变化主要遭受低灵敏度。近年来，基于靶向诱导的MNP数量变化的T-2-MRS具有较高的灵敏度，但这些传感器难以适应小分子。我们在此开发了一种超灵敏的T-2-MRS，其能够基于级联生物正交反应（BR）实现的MNP结合和组装来检测小分子。得益于快速和高选择性的级联BR，单个小分子靶标不仅可以增加MNP结合，而且可以组装MNP，这首次极大地放大了基于MNP的状态和数量变化的传感T-2信号。我们的策略是能够检测毒死蜱的线性范围为0.1 ng/mL至1000 ng/mL。通过对苹果和甘蓝样品中毒死蜱的检测，验证了该方法的实用性，其准确度高于酶联免疫吸附法。我们的测定提供了一种级联BR介导的MRS，其可以极大地拓宽基于T-2的MRS用于超灵敏地感测复杂样品中的痕量小分子的用途。
Conventional transverse relaxation time (T-2)-mediated magnetic resonance sensors (MRS) that utilizing the target-induces state change of magnetic nanoparticles (MNPs) mainly suffer from low sensitivity. Recent T-2-MRS that based on target-induced amount change of MNPs can achieve a higher sensitivity, but these sensors can hardly accommodate small molecules. We herein develop an ultrasensitive T-2-MRS that enable the detection of small molecules based on cascade bioorthogonal reactions (BRs)-realized MNPs binding and assembly. Benefiting from rapid and highly selective cascade BRs, a single small molecule target can not only increase MNPs binding but also assembly MNPs, which greatly amplifies T-2 signal for sensing based on both the state and amount change of MNPs for the first time. Our strategy is capable of sensing chlorpyrifos with a liner range of 0.1 ng/mL to 1000 ng/mL. We justify the practicability of our assay by detecting chlorpyrifos in apple and cabbage samples, whose accuracy is higher than that of enzyme linked immunosorbent assay. Our assay provides a cascade BRs-mediated MRS that can greatly broaden the use of T-2-based MRS for ultrasensitive sensing trace small molecules in complex samples.