Gut fungal dysbiosis and altered bacterial-fungal interaction in patients with diarrhea-predominant irritable bowel syndrome: An explorative study
Gut fungal dysbiosis and altered bacterial-fungal interaction in patients with diarrhea-predominant irritable bowel syndrome: An explorative study
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腹泻型肠易激综合征患者肠道真菌失调和细菌-真菌相互作用改变：一项探索性研究
DOI：
10.1111/nmo.13891
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发表时间：
2020-05-25
影响因子：
3.5
通讯作者：
Hou, Xiaohua
中科院分区：
文献类型：
--
作者：
Hong, Gaichao;Li, Ying;Hou, Xiaohua
背景IBS患者肠道真菌的研究较少，但对肠道菌群的研究已较多。方法选取符合罗马III诊断标准的55例以大肠杆菌为主的IBS（D-IBS）患者和16例健康对照者（HC）为研究对象。收集新鲜粪便样品并用于16 s rRNA和ITS 2高通量测序。D-IBS患者肠道细菌和真菌的多样性和组成，以及细菌-真菌相互作用的特点。LEfSe和RandomForest方法识别区分D-IBS和HC的特异性真菌类群，并通过斯皮尔曼相关性评估其与临床症状的相关性。结果与健康对照组相比，腹泻型肠易激综合征患者表现出异常（IBS-生态失调）或正常（HC-样IBS）粪便细菌结构和多样性。然而，粪便真菌特征在D-IBS和HC之间绝对不同，这表明D-IBS中肠道真菌比细菌更易发生变化。粪便真菌与IBS症状显著相关，特别是球腔菌属、曲霉属、孢子虫属和潘多拉属，它们被鉴定为可能区分D-IBS和HC。与HC相比，D-IBS患者肠道真菌相互作用明显减弱，其中念珠菌与细菌的相关性由负相关变为正相关，散囊菌属与细菌的相关性由正相关变为不相关，而德巴利酵母菌与细菌的相关性由负相关变为负相关。
Background Little is known about intestinal fungi in IBS patients whose gut bacteria have been investigated a lot. In order to explore causal relationship between IBS and gut mycobiome, and use gut fungi to diagnose or even treat IBS, further characterization of it in IBS is required.Methods Fifty-five diarrhea-predominant IBS (D-IBS) patients fulfilling Rome III criteria, and 16 healthy controls (HC) were recruited. Fresh fecal samples were collected and used for 16s rRNA and ITS2 high-throughput sequencing. Diversity and composition of gut bacteria and fungi, as well as bacterial-fungal interactions in D-IBS patients, were characterized. Specific fungal taxa differentiating D-IBS from HC were recognized by LEfSe and RandomForest methods, and their association with clinical symptoms was assessed by Spearman's correlation.Results Diarrhea-predominant irritable bowel syndrome patients showed abnormal (IBS-dysbiosis) or normal (HC-like IBS) fecal bacterial structure and diversity compared with healthy controls. However, fecal fungal signatures differed absolutely between D-IBS and HC, which indicated a more susceptible alteration of gut fungi than bacteria in D-IBS. Fecal fungi showed significant correlations with IBS symptoms, especially Mycosphaerella, Aspergillus, Sporidiobolus, and Pandora which were identified to potentially differentiate D-IBS from HC. Moreover, compared with HC there were markedly declined bacterial-fungal interactions in D-IBS, in which Candida changed from negative to positive correlations with bacteria, and Eurotium changed from positive correlations to irrelevance, while Debaryomyces gained negative correlations with bacteria.Conclusions Gut fungal dysbiosis and altered bacterial-fungal interactions were present in patients with D-IBS, and gut fungi could be used to diagnose D-IBS.