A NOVEL HIV-1 ISOLATE CONTAINING ALTERATIONS AFFECTING THE NF-KAPPA-B ELEMENT
A NOVEL HIV-1 ISOLATE CONTAINING ALTERATIONS AFFECTING THE NF-KAPPA-B ELEMENT
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DOI：
10.1016/0042-6822(91)90479-u
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发表时间：
1991-03-01
期刊：
影响因子：
3.7
通讯作者：
MARTIN, MA
中科院分区：
文献类型：
--
作者：
ENGLUND, G;HOGGAN, MD;MARTIN, MA
来自单个分离株（A1 -1）的三个HIV-1分子克隆在人T细胞系中增殖期间表现出不同的复制和致细胞病变特性。观察到的表型差异在很大程度上归因于影响病毒LTR的变化。核苷酸序列和PCR分析表明存在涉及NF-κB基序的新的重复或缺失。增强子元件的这些变化是在原始AL 1病毒储备液中鉴定的。将变体NF-κB片段亚克隆到LTR驱动的CAT表达载体中证实了启动子活性与复制/细胞病变能力之间的相关性。
Three molecular clones of HIV-1, derived from a single isolate (Al-1), exhibited distinct replicative and cytopathic properties during propagation in a human T cell line. The phenotypic differences observed were attributable, in large part, to changes affecting the viral LTR. Nucleotide sequence and PCR analyses demonstrated the presence of novel duplications or deletions involving the NF-κB motif. These changes in the enhancer element were identified in the original AL1 virus stock. Subcloning of the variant NF-κB segments into LTR-driven CAT expression vectors confirmed a correlation between promoter activity and replicative/cytopathic capacity.