Intelligent polymerized crystalline colloidal arrays: Novel chemical sensor materials
Intelligent polymerized crystalline colloidal arrays: Novel chemical sensor materials
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DOI：
10.1021/ac970853i
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发表时间：
1998-02-15
影响因子：
7.4
通讯作者：
Asher, SA
中科院分区：
文献类型：
--
作者：
Holtz, JH;Holtz, JSW;Asher, SA
我们报告了一种新的传感材料的发展，报告分析物的浓度，通过从聚合的结晶胶体阵列（PCCA）的可见光衍射。PCCA是在薄的智能聚合物水凝胶膜内聚合的球形聚苯乙烯胶体的介观周期性结晶胶体阵列（CCA）。CCA颜色鲜艳，并且它们有效地吸收满足布拉格条件的可见光。智能水凝胶包含化学分子识别剂，其导致凝胶响应于特定分析物的浓度而溶胀;凝胶体积是分析物浓度的函数。因此，从水凝胶膜衍射的颜色是分析物浓度的函数：凝胶的溶胀改变CCA的周期性，这导致衍射波长的偏移。我们已经制作了一个传感器，利用冠醚作为识别剂，检测Pb 2+在0.1 μ M-20 mM（类似于20 ppb-类似于4000 ppm）的浓度范围。我们还制作了葡萄糖和半乳糖传感器，利用葡萄糖氧化酶或β-D-半乳糖苷酶作为识别元件。葡萄糖氧化酶传感器在存在氧气的情况下检测0.1-0.5 mM（18-90 ppm）浓度范围内的葡萄糖，在不存在氧气的情况下检测低至10（-12）M葡萄糖（0.18 ppt）。此外，该传感器在恒定葡萄糖浓度的情况下报告近似1至6 ppm的溶解氧浓度。
We report the development of a novel sensing material that reports on analyte concentrations via diffraction of visible light from a polymerized crystalline colloidal array (PCCA). The PCCA is a mesoscopically periodic crystalline colloidal array (CCA) of spherical polystyrene colloids polymerized within a thin, intelligent polymer hydrogel film. CCAs are brightly colored, and they efficiently diffract visible light meeting the Bragg condition. The intelligent hydrogel incorporates chemical molecular recognition agents that cause the gel to swell in response to the concentration of particular analytes; the gel volume is a function of the analyte concentration. The color diffracted from the hydrogel film is, thus, a function of analyte concentration: the swelling of the gel changes the periodicity of the CCA, which results in a shift in the diffracted wavelength. We have fabricated a sensor, utilizing a crown ether as the recognition agent, that detects Pb2+ in the 0.1 mu M-20mM (similar to 20 ppb-similar to 4000 ppm) concentration range. We have also fabricated glucose and galactose sensors, utilizing glucose oxidase or beta-D-galactosidase as the recognition elements. The glucose oxidase sensor detects glucose in the 0.1-0.5 mM (18-90 ppm) concentration range in the presence of oxygen and detects as little as 10(-12) M glucose (0.18 ppt) in the absence of oxygen. In addition, this sensor reports on dissolved oxygen concentration from similar to 1 to 6 ppm in the presence of constant glucose concentrations.