A linkage map of the Asian tiger mosquito (Aedes albopictus) based on cDNA markers.
A linkage map of the Asian tiger mosquito (Aedes albopictus) based on cDNA markers.
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基于 cDNA 标记的亚洲虎蚊 (Aedes albopictus) 连锁图谱。
DOI：
10.1093/jhered/esq105
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发表时间：
2011
期刊：
影响因子：
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通讯作者：
Black4th,WilliamC
中科院分区：
文献类型：
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作者：
Sutherland,IanW;Mori,Akio;Montgomery,John;Fleming,KarenL;Anderson,JenniferM;Valenzuela,JesusG;Severson,DavidW;Black4th,WilliamC
亚洲虎蚊（Aedes（Stegomyia）albopictus（Skuse））是许多虫媒病毒的重要载体，并且种群在适应性特征方面表现出极端的变化，例如卵滞育、耐寒性和自生性（使一批卵成熟而不吸血的能力）。其中一些性状的遗传基础已经建立，但由于缺乏高分辨率的连锁图谱，无法对这些复杂性状的基因进行深入的遗传分析。本文报道了4个F_1作图家系的选育及其35个cDNA标记的定位结果。白纹伊蚊连锁图谱本研究增加了A.白纹伊蚊cDNA连锁图谱从38个增加到73个，标记密度从1个标记/5.7cM增加到1个标记/2.9cM，3个连锁群分别增加了9个、16个和10个标记。3个连锁群的总长度分别为64.5、76.5和71.6 cM，组合长度为212.6 cM。尽管保守的顺序中的大多数基因在4个家庭和以前的映射家庭，我们发现大量的异质性标记之间的重组量。这在连锁群I中最为显著，其在16.7和69.3 cM之间变化。一个地图整合的结果，从这4个家庭与早期的cDNA连锁图。
The Asian tiger mosquito,Aedes (Stegomyia) albopictus(Skuse), is an important vector of a number of arboviruses, and populations exhibit extreme variation in adaptive traits such as egg diapause, cold hardiness, and autogeny (ability to mature a batch of eggs without blood feeding). The genetic basis of some of these traits has been established, but lack of a high-resolution linkage map has prevented in-depth genetic analyses of the genes underlying these complex traits. We report here on the breeding of 4 F1intercross mapping families and the use of these to locate 35 cDNA markers to theA. albopictuslinkage map. The present study increases the number of markers on theA. albopictuscDNA linkage map from 38 to 73 and the density of markers from 1 marker/5.7 cM to 1 marker/2.9 cM and adds 9, 16, and 10 markers to the 3 linkage groups, respectively. The overall lengths of the 3 linkage groups are 64.5, 76.5, and 71.6 cM, respectively, for a combined length of 212.6 cM. Despite conservation in the order of most genes among the 4 families and a previous mapping family, we found substantial heterogeneity in the amount of recombination among markers. This was most marked in linkage group I, which varied between 16.7 and 69.3 cM. A map integrating the results from these 4 families with an earlier cDNA linkage map is presented.