Structure of liposome encapsulating proteins characterized by X-ray scattering and shell-modeling.
Structure of liposome encapsulating proteins characterized by X-ray scattering and shell-modeling.
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DOI：
10.1107/s0909049513020827
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发表时间：
2013-11
影响因子：
2.5
通讯作者：
Shimuzu N
中科院分区：
文献类型：
--
作者：
Hirai M;Kimura R;Takeuchi K;Hagiwara Y;Kawai-Hirai R;Ohta N;Igarashi N;Shimuzu N
使用第三代同步辐射光源的广角X射线散射数据。脂质体是有前途的药物递送系统，因为它们由于对活体的高度适应性而具有上级疗效。脂质脂质体包封蛋白质的构建和使用同步辐射小角和广角X-射线散射（SR-SWAXS）的结构检查。采用自然溶胀法、超声分散法、冻融法、挤出法和旋转过滤法制备脂质体。脂质体由酸性鞘糖脂（神经节苷脂）、胆固醇和磷脂组成。采用壳层模型法测定了脂质体的不对称双层结构和蛋白质的包封率。以及其他分析技术，SR-SWAXS和壳建模方法被证明是一个强大的工具，用于表征在原位结构的脂质体作为一个重要的候选药物输送系统。
Wide-angle X-ray scattering data using a third-generation synchrotron radiation source are presented. Lipid liposomes are promising drug delivery systems because they have superior curative effects owing to their high adaptability to a living body. Lipid liposomes encapsulating proteins were constructed and the structures examined using synchrotron radiation small- and wide-angle X-ray scattering (SR-SWAXS). The liposomes were prepared by a sequential combination of natural swelling, ultrasonic dispersion, freeze-throw, extrusion and spin-filtration. The liposomes were composed of acidic glycosphingolipid (ganglioside), cholesterol and phospholipids. By using shell-modeling methods, the asymmetric bilayer structure of the liposome and the encapsulation efficiency of proteins were determined. As well as other analytical techniques, SR-SWAXS and shell-modeling methods are shown to be a powerful tool for characterizing in situ structures of lipid liposomes as an important candidate of drug delivery systems.