Multipotential differentiation ability of GATA-1-null erythroid-committed cells
Multipotential differentiation ability of GATA-1-null erythroid-committed cells
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DOI：
10.1101/gad.1378206
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发表时间：
2006-03-15
影响因子：
10.5
通讯作者：
Nakano, T
中科院分区：
文献类型：
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作者：
Kitajima, K;Zheng, J;Nakano, T
加塔-1是一种锌指转录因子，被认为是原成红细胞生存所必需的。然而，我们发现，加塔-1-null proerythroblasts可以生存和增殖的OP 9基质细胞在促红细胞生成素的存在。此外，骨髓和肥大细胞诱导从加塔-1-null原成红细胞的刺激粒细胞-巨噬细胞集落刺激因子（GM-CSF）和白细胞介素-3（IL-3），分别，但淋巴样分化不能通过体内转移实现。因此，在没有终末分化所需的转录因子活性的情况下，即使相对成熟和定向的细胞也会连续增殖，并具有向其他谱系分化的能力。我们的数据表明，加塔-1是一个关键的转录因子，以固定红系祖细胞的红系谱系。
GATA-1, a zinc finger transcription factor, has been believed to be indispensable for the survival of proerythroblasts. However, we found that GATA-1-null proerythroblasts could survive and proliferate on OP9 stroma cells in the presence of erythropoietin. Furthermore, myeloid and mast cells were induced from the GATA-1-null proerythroblasts by the stimulation of granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-3 (IL-3), respectively, but lymphoid differentiation was not achieved by in vivo transfer. Thus, without activity of the transcription factor required for terminal differentiation, even relatively mature and committed cells proliferate continuously with the differentiation capacity to other lineages. Our data suggest that GATA-1 is a critical transcription factor to fix erythroid progenitors to the erythroid lineage.