Impact of wheat straw decomposition on successional patterns of soil microbial community structure
Impact of wheat straw decomposition on successional patterns of soil microbial community structure
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DOI：
10.1016/j.soilbio.2008.10.024
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发表时间：
2009-02-01
影响因子：
9.7
通讯作者：
Ranjard, Lionel
中科院分区：
文献类型：
--
作者：
Bastian, Fabiola;Bouziri, Lamia;Ranjard, Lionel
研究了小麦秸秆残体腐解过程中土壤土著细菌和真菌群落的动态变化。更确切地说，这种动态的影响下，在不同的土壤区的分解麦秸残留物（即残留物，土壤相邻的残留物= detritusbium，和散装土壤）。通过对来自这些不同区域的DNA提取物应用B-和F-ARISA（用于细菌和真菌自动化核糖体基因间间隔区分析），在整个分解过程中比较细菌和真菌群落的遗传结构。残体分解引起土壤细菌和真菌群落动态的显著变化，不同土壤带间变化的大小顺序为残体>碎屑土>非残体土，证实了残体影响范围在与残体接触的4-6 mm土壤带内的空间结构。此外，显着差异的细菌和真菌群落结构之间的早期（14和28天）和后期（56至168天）的分解阶段是明显的。这些可能与生态属性，如继承的r-（copiotrophs）和K-（寡养）的战略家。在早期（28天）和晚期（168天）降解阶段的微生物多样性进一步分析的残留区的DNA提取物中的16 S和18 S rDNA的分子库存。这证实了不同种群在残留物分解过程中的演替。荧光假单胞菌属和脉孢菌属（Neurospora sp.）在早期占优势，随后受到放线菌门（Actinobacteria）和三角形变形菌门（Deltaproteobacteria）分类群以及担子菌门（Basidiomycota）真菌分类群和马杜雷氏菌属（Madurella spp.）根据这些人口的生态属性，在土壤中的新鲜有机残留物的微生物演替将占主导地位的copiotrophs和r-战略家在早期阶段，与寡养（K-战略家）的相对丰度增加基板数量和/或质量随时间的推移下降。(C)2008爱思唯尔有限公司保留所有权利。
The dynamics of indigenous bacterial and fungal soil communities were followed throughout the decomposition of wheat straw residue. More precisely, such dynamics were investigated in the different soil zones under the influence of decomposing wheat straw residue (i.e. residues, soil adjacent to residue = detritusphere, and bulk soil). The genetic structures of bacterial and fungal communities were compared throughout the decomposition process long by applying B- and F-ARISA (for bacterial and fungal-automated ribosomal intergenic spacer analysis) to DNA extracts from these different zones. Residue decomposition induced significant changes in bacterial and fungal community dynamics with a magnitude of changes between the different soil zones ordered as followed: residue > detritusphere > bulk soil, confirming the spatial structuration of the sphere of residue influence to the 4-6 mm soil zone in contact with residue. Furthermore, significant differences in the structure of bacterial and fungal communities were apparent between the early (14 and 28 days) and late (from 56 to 168 days) stages of decomposition. These could be related to ecological attributes such as the succession of r- (copiotrophs) and K- (oligotrophs) strategists. Microbial diversity at the early (28 days) and late (168 days) stages of degradation was further analysed by a molecular inventory of 16S and 18S rDNA in DNA extracts from the residue zone. This confirmed the succession of different populations during residue decomposition. Fluorescent Pseudomonas spp. and Neurospora sp. were dominant in the early stage with subsequent stimulation of Actinobacteria and Deltaproteobacteria taxa, as well as Basidiomycota fungal taxa and Madurella spp. According to the ecological attributes of these populations, microbial succession on fresh organic residue incorporated in soil would be dominated by copiotrophs and r-strategists in the early stages, with oligotrophs (K-strategists) increasing in relative abundance as substrate quantity and/or quality declines over time. (C) 2008 Elsevier Ltd. All rights reserved.