Cortex Mori Radicis extract exerts antiasthmatic effects via enhancement of CD4+CD25+Foxp3+ regulatory T cells and inhibition of Th2 cytokines in a mouse asthma model
Cortex Mori Radicis extract exerts antiasthmatic effects via enhancement of CD4+CD25+Foxp3+ regulatory T cells and inhibition of Th2 cytokines in a mouse asthma model
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DOI：
10.1016/j.jep.2011.08.021
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发表时间：
2011-10-31
影响因子：
5.4
通讯作者：
Park, Eun-Jung
中科院分区：
文献类型：
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作者：
Kim, Hyun-Ji;Lee, Hai Ja;Park, Eun-Jung
民族药理学相关性：桑根皮质（CMR）是桑的根表皮，传统上在东方医学中用于治疗咳嗽。本研究在小鼠哮喘模型中探讨CMR抑制气道高反应性（AHR）的免疫学机制。材料与方法：采用卵清蛋白致敏Balb/c小鼠，建立实验性哮喘模型，然后进行气溶胶致敏反应。CMR（50或200 mg/kg）在OVA致敏后3周开始口服，持续6周。采用流式细胞术、酶联免疫吸附试验（ELISA）和逆转录聚合酶链反应（RT-PCR）检测AHR、肺嗜酸性粒细胞积累、免疫球蛋白E （IgE）、组胺、Th2细胞因子表达和CD4(+)CD25(+) foxxp（3+）调节性T细胞（Tregs）。结果：CMR显著降低AHR反应、嗜酸性粒细胞浸润、血清组胺和ova特异性IgE的产生。此外，CMR在蛋白（分泌）和mRNA水平上抑制Th2细胞因子，如白细胞介素(IL)-4、-5和-13。值得注意的是，CMR在小鼠哮喘模型中显著增加Foxp3(+) Tregs种群和Foxp3(+) mRNA表达。结论：CMR是一种有效的抗哮喘药物，通过增强小鼠哮喘模型中CD4(+)CD25(+)Foxp3（+）调节性T细胞和抑制Th2细胞因子发挥抗过敏作用。2011爱思唯尔爱尔兰有限公司版权所有。
Ethonopharmacological relevance: Cortex Mori Radicis (CMR), the root epidermis of Morus alba L, has been traditionally used for cough treatment in Oriental medicine. In the present study, immunological mechanism of CMR in inhibition of airway hyperresponsiveness (AHR) was investigated in a mouse asthma model.Materials and methods: Experimental asthma model was established in Balb/c mice sensitized by ovalbumin (OVA), followed by aerosol allergen challenges. CMR (50 or 200 mg/kg) was orally administered for 6-weeks from 3-weeks after OVA sensitization. AHR, pulmonary eosinophilic accumulation, immunoglobulin E (IgE), histamine, Th2 cytokine expression, and CD4(+)CD25(+)Foxp(3+) regulatory T cells (Tregs) were evaluated by flow cytometry, enzyme-linked immunosorbent assay (ELISA) and reverse transcription-polymerase chain reaction (RT-PCR).Results: CMR significantly reduced AHR response, eosinophil infiltration, and production of serum histamine and OVA-specific IgE. Furthermore, CMR suppressed Th2 cytokines such as interleukin (IL)-4, -5 and -13 at protein (secreted) and mRNA levels. Of note, CMR significantly increased Foxp3(+) Tregs population and enhanced Foxp3(+) mRNA expression in a mouse asthma model.Conclusions: CMR exerts anti-allergic effect via enhancement of CD4(+)CD25(+)Foxp3(+) regulatory T cells and inhibition of Th2 cytokines in a mouse asthma model as a potent anti-asthmatic agent. (C) 2011 Elsevier Ireland Ltd. All rights reserved.