Effects of Human Fecal Flora on Intestinal Morphology and Mucosal Immunity in Human Flora-associated Piglet
Effects of Human Fecal Flora on Intestinal Morphology and Mucosal Immunity in Human Flora-associated Piglet
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DOI：
10.1111/j.1365-3083.2008.02211.x
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发表时间：
2009-03-01
影响因子：
3.7
通讯作者：
Yang, Q.
中科院分区：
文献类型：
--
作者：
Che, C.;Pang, X.;Yang, Q.
建立人菌群相关(HFA)仔猪模型，研究来自不同供体物种的肠道微生物对仔猪肠道形态和粘膜免疫的影响。将经剖宫产获得的无菌新生仔猪分别经口接种人和猪粪便悬液，人工饲养，分别建立HFA组(n=7)和猪菌群相关组(PFA)。6周后处死所有猪。采集十二指肠、空肠、回肠和结肠组织标本，采用组织化学和免疫组织化学方法进行肠道形态分析和免疫活性细胞检测。总而言之，两组猪的表现都很好，但HFA猪的日增重略好一些，它们的空肠绒毛高度和隐窝深度明显更高。与PFA猪相比，HFA猪空肠上皮内淋巴细胞数量减少，但含有中性粘蛋白的杯状细胞数量显著增加。肥大细胞数量无明显差异。HFA猪空肠中的IgA产生细胞和CD4(+)T细胞的面积以及小肠中的IgG产生细胞的面积显著高于HFA猪。而十二指肠和结肠中MHC-II类表达细胞的面积显著增加。此外，还观察了双歧杆菌的数量。在HFA猪中显著较高。本研究证实肠道微生物的组成对宿主肠道黏膜免疫功能有不同程度的影响，并提示共生细菌对肠道的健康和发育有重要影响。
Human flora-associated (HFA) piglet model was established to examine the effects of gut microbes from a different donor species on the intestinal morphology and mucosal immunity. Newborn germ-free piglets, obtained by caesarean section, were orally inoculated with a human and a porcine faecal suspension, and artificially fed to establish a HFA group (n = 7) and pig flora-associated (PFA) group (n = 7), respectively. All pigs were killed 6 weeks later. Tissue samples from duodenum, jejunum, ileum and colon were collected and studied by histochemistry and immunohistochemistry methods for intestinal morphological analyses and detection of immunocompetent cells. In summary, both groups of pigs performed well but HFA pigs had a somewhat better daily weight gain, and their jejunal villus height and crypt depth were significantly higher. In comparison with PFA pigs, the number of intraepithelial lymphocytes in jejunum was lower but the number of goblet cells containing neutral mucins was significantly increased in HFA pigs. No difference was observed in the number of mast cells. The areas of IgA producing cells and CD4(+) T cells in the jejunum and IgG producing cells in the small intestine were significantly higher in HFA pigs. However, the areas of MHC class II expressing cells were significantly increased in the duodenum and colon. Additionally, the amount of Bifidobacteria spp. was significantly higher in HFA pigs. This study confirms that the composition of gut microbes differentially affects the host intestinal mucosal immunity and suggests that commensal bacteria have great effects on intestinal health and development.